Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol
文献类型：期刊论文
| 作者 | Liu, Ying; Guo, Chen; Liu, Chun-Zhao1 |
| 刊名 | CHIRALITY
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 27期号:3页码:199-204 |
| 关键词 | Yarrowia lipolytica lipase magnetic nanoparticles cross-linked enzyme aggregates immobilization (R S)-2-octanol enantioselectivity |
| ISSN号 | 0899-0042 |
| 其他题名 | Chirality |
| 中文摘要 | Novel magnetic cross-linked lipase aggregates were fabricated by immobilizing the cross-linked lipase aggregates onto magnetic particles with a high number of -NH2 terminal groups using p-benzoquinone as the cross-linking agent. At the optimal fabrication conditions, 100% of immobilization efficiency and 139% of activity recovery of the magnetic cross-linked lipase aggregates were achieved. The magnetic cross-linked lipase aggregates were able to efficiently resolve (R, S)-2-octanol, and retained 100% activity and 100% enantioselectivity after 10cycles of reuse, whereas the cross-linked lipase aggregates only retained about 50% activity and 70% enantioselectivity due to insufficient cross-linking. These results provide a great potential for industrial applications of the magnetic cross-linked lipase aggregates. Chirality 27:199-204, 2015. (c) 2014 Wiley Periodicals, Inc. |
| 英文摘要 | Novel magnetic cross-linked lipase aggregates were fabricated by immobilizing the cross-linked lipase aggregates onto magnetic particles with a high number of -NH2 terminal groups using p-benzoquinone as the cross-linking agent. At the optimal fabrication conditions, 100% of immobilization efficiency and 139% of activity recovery of the magnetic cross-linked lipase aggregates were achieved. The magnetic cross-linked lipase aggregates were able to efficiently resolve (R, S)-2-octanol, and retained 100% activity and 100% enantioselectivity after 10cycles of reuse, whereas the cross-linked lipase aggregates only retained about 50% activity and 70% enantioselectivity due to insufficient cross-linking. These results provide a great potential for industrial applications of the magnetic cross-linked lipase aggregates. Chirality 27:199-204, 2015. (c) 2014 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Chemistry, Medicinal ; Chemistry, Analytical ; Chemistry, Organic ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Chemistry |
| 关键词[WOS] | OPTIMUM PERFORMANCE ; CLEAS ; IMMOBILIZATION ; BIOCATALYSTS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000350137200003 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11798]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Guo, Chen,Liu, Chun-Zhao. Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol[J]. CHIRALITY,2015,27(3):199-204. |
| APA | Liu, Ying,Guo, Chen,&Liu, Chun-Zhao.(2015).Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol.CHIRALITY,27(3),199-204. |
| MLA | Liu, Ying,et al."Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol".CHIRALITY 27.3(2015):199-204. |
入库方式： OAI收割
来源：过程工程研究所
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