Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens
文献类型：期刊论文
| 作者 | Liu, Yuan1,2; Wang, Lianyan1; Zhang, Yu3; Zhang, Weifeng1,2; Chen, Xiaoming1,2; Yang, Tingyuan1; Wang, Zhuo3; Ma, Guanghui1 |
| 刊名 | INTERNATIONAL IMMUNOPHARMACOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 23期号:2页码:603-608 |
| 关键词 | Emulsion Immune response Membrane emulsification Size distribution Vaccine |
| ISSN号 | 1567-5769 |
| 其他题名 | Int. Immunopharmacol. |
| 中文摘要 | Water-in-oil (W/O) emulsion is one of the best "one-shot" delivery system for antigen to generate high and durable antibody response. Here a facile method using premixed Shirasu Porous Glass (SPG) membrane emulsification technique was employed to fabricate uniform-sized emulsion and mechanical stirring was employed to prepare non-uniform-sized emulsion as control. In release kinetics studies, uniform-sized emulsion showed low initial release, and during the followed 17 days, uniform-sized emulsions released antigen faster than emulsions of non-uniform size. In addition, vaccination studies against Newcastle disease virus (NDV) and avian Influenza virus (AIV) demonstrated that emulsion of uniform size induced higher HI antibodies and antigen-specific IgG titers. Furthermore, chickens vaccinated with emulsion of uniform size had a significantly greater ratio of CD8(+)T cells to CD4(+)T cells and a higher percentage of CD8(+)CD4(+)T cells. Taken together, these results indicated that emulsion of uniform size induced a more potent immune response than emulsion prepared by mechanical stirring, and warranted the use of SPG membrane emulsification technique in generating new avian vaccines. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | Water-in-oil (W/O) emulsion is one of the best "one-shot" delivery system for antigen to generate high and durable antibody response. Here a facile method using premixed Shirasu Porous Glass (SPG) membrane emulsification technique was employed to fabricate uniform-sized emulsion and mechanical stirring was employed to prepare non-uniform-sized emulsion as control. In release kinetics studies, uniform-sized emulsion showed low initial release, and during the followed 17 days, uniform-sized emulsions released antigen faster than emulsions of non-uniform size. In addition, vaccination studies against Newcastle disease virus (NDV) and avian Influenza virus (AIV) demonstrated that emulsion of uniform size induced higher HI antibodies and antigen-specific IgG titers. Furthermore, chickens vaccinated with emulsion of uniform size had a significantly greater ratio of CD8(+)T cells to CD4(+)T cells and a higher percentage of CD8(+)CD4(+)T cells. Taken together, these results indicated that emulsion of uniform size induced a more potent immune response than emulsion prepared by mechanical stirring, and warranted the use of SPG membrane emulsification technique in generating new avian vaccines. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Immunology ; Pharmacology & Pharmacy |
| 关键词[WOS] | ADJUVANT ACTIVITY ; T-CELLS ; RELEASE ; EFFICACY ; POLYSACCHARIDES ; NANOPARTICLES ; PARTICLES ; LIPOSOME ; KINETICS ; IMPROVE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000346954000032 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11799]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad ofSciences, Inst Proc Engn, PIA Key Lab Biopharmaceut Prod & Formulat Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Sinder Weite Technol Co Ltd, Beijing 101302, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Wang, Lianyan,Zhang, Yu,et al. Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2014,23(2):603-608. |
| APA | Liu, Yuan.,Wang, Lianyan.,Zhang, Yu.,Zhang, Weifeng.,Chen, Xiaoming.,...&Ma, Guanghui.(2014).Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens.INTERNATIONAL IMMUNOPHARMACOLOGY,23(2),603-608. |
| MLA | Liu, Yuan,et al."Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens".INTERNATIONAL IMMUNOPHARMACOLOGY 23.2(2014):603-608. |
入库方式： OAI收割
来源：过程工程研究所
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