Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
文献类型：期刊论文
| 作者 | Wang, Honghui1; Pan, Jiachuan2; Wang, Jing1; Wang, Nan1; Zhang, Jie1; Li, Qiang3; Wang, Dan1; Zhou, Xiaohua1 |
| 刊名 | BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
 |
| 出版日期 | 2014-11-02 |
| 卷号 | 28期号:6页码:1042-1049 |
| 关键词 | response surface methodology succinic acid fermentation Escherichia coli xylose mother liquor |
| ISSN号 | 1310-2818 |
| 其他题名 | Biotechnol. Biotechnol. Equip. |
| 中文摘要 | Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L-1 total initial sugars, 42.27 g L-1 MgCO3 and 17.84 g L-1 yeast extract. The maximum production of succinic acid was 52.09 +/- 0.21 g L-1 after 84h with a yield of 0.63 +/- 0.03 g g(-1) total sugar, approaching the predicted value (53.18 g L-1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. |
| 英文摘要 | Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L-1 total initial sugars, 42.27 g L-1 MgCO3 and 17.84 g L-1 yeast extract. The maximum production of succinic acid was 52.09 +/- 0.21 g L-1 after 84h with a yield of 0.63 +/- 0.03 g g(-1) total sugar, approaching the predicted value (53.18 g L-1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; ALKALINE PROTEASE PRODUCTION ; ACTINOBACILLUS-SUCCINOGENES ; ANAEROBIC CONDITIONS ; RENEWABLE RESOURCES ; BOVINE RUMEN ; BACILLUS SP ; FERMENTATION ; OPTIMIZATION ; HYDROLYSATE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000348931600008 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11836]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China 2.Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Honghui,Pan, Jiachuan,Wang, Jing,et al. Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2014,28(6):1042-1049. |
| APA | Wang, Honghui.,Pan, Jiachuan.,Wang, Jing.,Wang, Nan.,Zhang, Jie.,...&Zhou, Xiaohua.(2014).Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,28(6),1042-1049. |
| MLA | Wang, Honghui,et al."Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 28.6(2014):1042-1049. |
入库方式： OAI收割
来源：过程工程研究所
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