Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii
文献类型：期刊论文
| 作者 | Wang, P.; Zhang, Z. W.; Jiao, Y. J.; Liu, S. X.; Wang, Y. S. |
| 刊名 | Journal of Biotechnology
 |
| 出版日期 | 2015 |
| 期号 | JAN页码:123-129 |
| 关键词 | Vitamin B12 Propionic acid 5 Feed back inhibition Expanded bed adsorption bioreactor PSEUDOMONAS-DENITRIFICANS WHEAT-STRAW BIOSYNTHESIS ENZYME 6-Dimethylbenzimidazole |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | An efficient fermentation-strengthening approach was developed to improve the anaerobic production of vitamin B12 by cultivation process optimization with Propionibacterium freudenreichit The effects of the byproduct propionic acid and the precursor 5,6-dimethylbenzimidazole (DMB) on vitamin B12 biosynthesis were investigated. Byproduct inhibition experiments showed that maintaining propionic acid concentration in broth below 10-20 g/L in the early stage and 20-30 g/L in the late stage can efficiently improve vitamin B12 biosynthesis. Batch fermentation indicated the occurrence of feed-back inhibition in intracellular intermediate biosynthesis. In addition, the incorporation of the precursor DMB depended on the fermentation level of the vitamin B12 intermediate. High vitamin B12 concentration (58.8 mg/L) and production (0.37 mg/g) were obtained with an expanded bed adsorption bioreactor by using the propionic acid and DMB control method. The optimum concentration and production of 59.5 and 0.59 mg/L h for vitamin B12 production were respectively achieved after five continuous batches. (C) 2014 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000346826800020 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11844]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Wang, P.,Zhang, Z. W.,Jiao, Y. J.,et al. Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii[J]. Journal of Biotechnology,2015(JAN):123-129. |
| APA | Wang, P.,Zhang, Z. W.,Jiao, Y. J.,Liu, S. X.,&Wang, Y. S..(2015).Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii.Journal of Biotechnology(JAN),123-129. |
| MLA | Wang, P.,et al."Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii".Journal of Biotechnology .JAN(2015):123-129. |
入库方式： OAI收割
来源：过程工程研究所
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