CO binding improves the structural, functional, physical and anti-oxidation properties of the PEGylated hemoglobin
文献类型：期刊论文
| 作者 | Wang, Q. Q.; Hu, T.; Sun, L. J.; Ji, S. Y.; Zhao, D. W.; Liu, J. X.; Ma, G. H.; Su, Z. G. |
| 刊名 | Artificial Cells Nanomedicine and Biotechnology
 |
| 出版日期 | 2015 |
| 期号 | 1页码:18-25 |
| 关键词 | autoxidation heme environment oxygen carrier PEGylation thermal stability CARBONMONOXY HEMOGLOBIN CIRCULAR-DICHROISM IN-VITRO PEG-HB DISSOCIATION CONJUGATE MP4 CARRIER LINKER SITES |
| 其他题名 | Artif. Cell. Nanomed. Biotechnol. |
| 中文摘要 | Context: PEGylated hemoglobin (Hb) is a promising oxygen therapeutic agent for clinical application. However, it suffered from structural perturbation, functional instability and methemoglobin (metHb) formation. Objective: To improve the structural, functional, physical and anti-oxidation properties of the PEGylated Hb. Materials and methods: PEGylation of Hb with CO binding (HbCO) was conducted using maleimide and acylation chemistry, respectively. Physical and chemical parameters were measured for Hb samples. The circular dichroism spectra, dynamic light scattering and analytical ultracentrifugation were used to investigate the structure and conformation of PEGylated HbCO. Results: CO binding can inhibit the autoxidation of the PEGylated Hb, structurally stabilize its tetramer and improve its thermal and pH stability. Importantly, the circular dichroism spectra showed that CO binding can decrease the structural perturbation of Hb induced by PEGylation. The PEGylated HbCO with CO release showed slightly higher oxygen-delivery capacity than the PEGylated Hb. The PEGylated HbCO did not show metHb formation after 30-day storage at 4 degrees C. Discussion and conclusion: CO binding structurally stabilized the PEGylated Hb, abolished its metHb formation, and significantly increased its physical stability. In particular, it also avoided the perturbation of PEG chains on the heme microenvironment. The functional property of the PEGylated HbCO can be maintained during its long-term storage, which is of great significance for field transfusion. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000347531900003 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11847]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Wang, Q. Q.,Hu, T.,Sun, L. J.,et al. CO binding improves the structural, functional, physical and anti-oxidation properties of the PEGylated hemoglobin[J]. Artificial Cells Nanomedicine and Biotechnology,2015(1):18-25. |
| APA | Wang, Q. Q..,Hu, T..,Sun, L. J..,Ji, S. Y..,Zhao, D. W..,...&Su, Z. G..(2015).CO binding improves the structural, functional, physical and anti-oxidation properties of the PEGylated hemoglobin.Artificial Cells Nanomedicine and Biotechnology(1),18-25. |
| MLA | Wang, Q. Q.,et al."CO binding improves the structural, functional, physical and anti-oxidation properties of the PEGylated hemoglobin".Artificial Cells Nanomedicine and Biotechnology .1(2015):18-25. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。