A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles
文献类型：期刊论文
| 作者 | Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xia, Tingting1; Xiong, Wubin1; Guo, Chen3 |
| 刊名 | MATERIALS LETTERS
 |
| 出版日期 | 2014-12-15 |
| 卷号 | 137期号:DEC.页码:113-116 |
| ISSN号 | 0167-577X |
| 关键词 | Magnetic materials Biomaterials Nanoparticles Mycoplasma pneumonia |
| 其他题名 | Mater. Lett. |
| 中文摘要 | The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles (MNPs) was determined and compared with whole Fe3O4 MNPs in this study. We detected the peroxidase-like activity of Fe3O4 MNPs through the catalytic reaction between Fe3O4 MNPs and TMB. Due to the high catalytic activity of ferric ions and ferrous ions released from Fe3O4 MNPs after dissolution, dissolved Fe3O4 MNPs showed much better peroxidase-like activity than whole Fe3O4 MNPs. In addition, we used the peroxidase-like activity of Fe3O4 MNPs in the diagnosis of mycoplasma pneumonia infection in which Fe3O4 MNPs was used as substitution of horseradish peroxidase. Both Fe3O4 MNPs modified with amino groups (MNPs-NH2) and Fe3O4 MNPs modified with polyethyleneimine (MNPs-PEI) were successfully used in the indirect immunoassay for the detection of mycoplasma pneumoniae. And the results of dissolved Fe3O4 MNPs were more sensitive and specific than that of whole Fe3O4 MNPs. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles (MNPs) was determined and compared with whole Fe3O4 MNPs in this study. We detected the peroxidase-like activity of Fe3O4 MNPs through the catalytic reaction between Fe3O4 MNPs and TMB. Due to the high catalytic activity of ferric ions and ferrous ions released from Fe3O4 MNPs after dissolution, dissolved Fe3O4 MNPs showed much better peroxidase-like activity than whole Fe3O4 MNPs. In addition, we used the peroxidase-like activity of Fe3O4 MNPs in the diagnosis of mycoplasma pneumonia infection in which Fe3O4 MNPs was used as substitution of horseradish peroxidase. Both Fe3O4 MNPs modified with amino groups (MNPs-NH2) and Fe3O4 MNPs modified with polyethyleneimine (MNPs-PEI) were successfully used in the indirect immunoassay for the detection of mycoplasma pneumoniae. And the results of dissolved Fe3O4 MNPs were more sensitive and specific than that of whole Fe3O4 MNPs. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Physics, Applied |
| 研究领域[WOS] | Materials Science ; Physics |
| 关键词[WOS] | PEROXIDASE-LIKE ACTIVITY ; C-REACTIVE PROTEIN ; MAGNETIC NANOPARTICLES ; HRP ; PARTICLES |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0167577X14016127 |
| 语种 | 英语 |
| WOS记录号 | WOS:000345469700031 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11870]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles[J]. MATERIALS LETTERS,2014,137(DEC.):113-116. |
| APA | Yang, Mingzhu,Guan, Yueping,Yang, Yu,Xia, Tingting,Xiong, Wubin,&Guo, Chen.(2014).A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles.MATERIALS LETTERS,137(DEC.),113-116. |
| MLA | Yang, Mingzhu,et al."A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles".MATERIALS LETTERS 137.DEC.(2014):113-116. |
入库方式： OAI收割
来源：过程工程研究所
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