Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants
文献类型：期刊论文
| 作者 | Zhao, Y.1,3; Stiles, A. R.1; Saxena, P. K.2; Liu, C. Z.1 |
| 刊名 | BIOLOGIA PLANTARUM
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 57期号:1页码:189-192 |
| 关键词 | auxin cytokinin gibberellic acid polyphenol oxidase |
| ISSN号 | 0006-3134 |
| 英文摘要 | An efficient in vitro plant regeneration system has been developed using dark preincubated leaf explants of Rhodiola crenulata, a traditional Tibetan medicinal plant. The leaf explants, preincubated in the dark for 5 d, developed an average of 9.1 shoots per explant on a medium containing 15 mu M N (6)-benzyladenine (BA) and 2.5 mu M gibberellic acid (GA(3)). The biochemical mechanism underlying dark-induced shoot organogenesis was investigated by measuring polyphenol oxidase (PPO) activity. Dark preincubation significantly reduced PPO activity in leaf explants during the initial period of shoot organogenesis and reduced browning compared to explants cultured in the light. Up to 88.4 % of the regenerated shoots formed roots and developed into complete plantlets on a medium containing 5 mu M indoleacetic acid (IAA) within 25 d. Approximately 82 % of the regenerated plantlets survived transplantation and grew vigorously in the greenhouse. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | IN-VITRO ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; TISSUE-CULTURES ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318647800029 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13316]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Y.,Stiles, A. R.,Saxena, P. K.,et al. Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants[J]. BIOLOGIA PLANTARUM,2013,57(1):189-192. |
| APA | Zhao, Y.,Stiles, A. R.,Saxena, P. K.,&Liu, C. Z..(2013).Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants.BIOLOGIA PLANTARUM,57(1),189-192. |
| MLA | Zhao, Y.,et al."Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants".BIOLOGIA PLANTARUM 57.1(2013):189-192. |
入库方式： OAI收割
来源：过程工程研究所
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