Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation
文献类型：期刊论文
| 作者 | Li, Yanjun1,2; Peng, Xiaowei1; Chen, Hongzhang1
|
| 刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 116期号:4页码:493-498 |
| 关键词 | Lignocellulose Fungal secretome Cellulase activity Solid-state fermentation Submerged fermentation |
| ISSN号 | 1389-1723 |
| 英文摘要 | Although submerged fermentation (SmF) accounts for most of current enzyme industries, it has been reported that solid-state fermentation (SSF) can produce higher enzyme yields in laboratory scale. In order to understand the reasons contributing to high enzyme production in SSF, this study compared the cellulase activities and secretomes of Neurospora sitophila cultured in SSF and SmF using steam exploded wheat straw as carbon source and enzyme inducer. The total amounts of protein and biomass (glucosamine content) in SSF were respectively 30 and 2.8 times of those in SmF. The CMCase, FPA and beta-glucoside activities in SSF were 53-181 times of those in SmF. Both in SSF and SmF, N. sitophila secreted the most critical cellulases and hemicellulases known for Trichoderma reesei, although a beta-xylosidase was exclusively identified in SSF. Six endoglucanases were identified in N. sitophila secretion with the high CMCase activity. The non-enzyme proteins in SSF were involved in fungal mycelia growth and conidiation; while those in SmF were more related to glycometabolism and stress tolerance. This revealed that SSF more likely serves as a natural habitat for filamentous fungi to facilitate the enzyme secretion. (C) 2013, The Society for Biotechnology, Japan. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 关键词[WOS] | TRICHODERMA-REESEI ; FILAMENTOUS FUNGUS ; TRANSCRIPTIONAL REGULATION ; EXTRACELLULAR PROTEINS ; CELLULASE PRODUCTION ; ENZYME-PRODUCTION ; CARBON SOURCE ; CRASSA ; HEMICELLULASES ; CONIDIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326559500014 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13318]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yanjun,Peng, Xiaowei,Chen, Hongzhang. Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2013,116(4):493-498. |
| APA | Li, Yanjun,Peng, Xiaowei,&Chen, Hongzhang.(2013).Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,116(4),493-498. |
| MLA | Li, Yanjun,et al."Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 116.4(2013):493-498. |
入库方式： OAI收割
来源：过程工程研究所
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