Isotherm Type Shift of Hydrophobic Interaction Adsorption and its Effect on Chromatographic Behavior
文献类型：期刊论文
| 作者 | Meng, Qingqiang1,2; Wang, Jiaxing1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 51期号:2页码:173-180 |
| ISSN号 | 0021-9665 |
| 英文摘要 | Adsorption isotherm type is a key factor in simulating chromatographic profiles. In this study, the adsorption isotherm type of pure bovine serum albumin (BSA) and immunoglobulin G (IgG) on hydrophobic interaction chromatography (HIC) media was found to shift from Freundlich to Langmuir with the increase of salt concentration. For BSA on butyl-, phenyl- and octyl-sepharose, the isotherm type shift occurred when the salt concentrations were higher than 1.8, 1.5 and 1.4 mol/L, respectively. For IgG, the turning points were 1.0 mol/L on phenyl-sepharose and 0.9 mol/L on octyl-sepharose. Circular dichroism spectra and high-performance size-exclusion chromatography found no obvious conformational change or oligomer formation for the proteins in the solutions with different salt concentrations. HIC profiles of BSA and IgG revealed that the isotherm type shift greatly affected the chromatographic behavior, because the original single peak was coincidentally split into two peaks at the salt concentrations over which the isotherm type shift occurred. Combining both the isotherm type shift and peak-splitting phenomena, it was possible that the change of protein protein repulsion among adsorbed protein molecules under different salt concentrations caused the abnormal behavior of adsorption isotherm and chromatographic profiles. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ALPHA-LACTALBUMIN ; PROTEINS ; SALT ; RETENTION ; SURFACES ; TEMPERATURE ; ADSORBENTS ; EQUATION ; COLUMN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315172100013 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13319]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Qingqiang,Wang, Jiaxing,Ma, Guanghui,et al. Isotherm Type Shift of Hydrophobic Interaction Adsorption and its Effect on Chromatographic Behavior[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2013,51(2):173-180. |
| APA | Meng, Qingqiang,Wang, Jiaxing,Ma, Guanghui,&Su, Zhiguo.(2013).Isotherm Type Shift of Hydrophobic Interaction Adsorption and its Effect on Chromatographic Behavior.JOURNAL OF CHROMATOGRAPHIC SCIENCE,51(2),173-180. |
| MLA | Meng, Qingqiang,et al."Isotherm Type Shift of Hydrophobic Interaction Adsorption and its Effect on Chromatographic Behavior".JOURNAL OF CHROMATOGRAPHIC SCIENCE 51.2(2013):173-180. |
入库方式： OAI收割
来源：过程工程研究所
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