Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles
文献类型：期刊论文
| 作者 | Zheng, Muqing1; Su, Zhiguo1; Ji, Xiaoyuan1; Ma, Guanghui1; Wang, Ping2,3; Zhang, Songping1 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2013-10-20 |
| 卷号 | 168期号:2页码:212-217 |
| 关键词 | Multienzyme Magnetic nanoparticles Cofactor regeneration Magnetic field Immobilization |
| ISSN号 | 0168-1656 |
| 英文摘要 | Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating magnetic field on magnetic nanoparticles-supported multi-enzymatic system so that the inter-particle collisions due to Brownian motion of nanoparticles could be improved. To that end, a multienzyme system including glutamate dehydrogenase (GluDH), glucose dehydrogenase (GDH) and cofactor NAD(H) were separately immobilized on silica coated Fe3O4 magnetic nanoparticles with an average diameter of 105 nm, and the effect of magnetic field strength and frequency on the kinetics of the coupled bi-enzyme reaction was investigated. It was found that at low magnetic field frequency (25 Hz and 100 Hz), increasing magnetic field strength from 9.8 to 161.1 Gs led to only very slight increase in reaction rate of the coupled bi-enzyme reaction expressed by glucose consumption rate. At higher magnetic field of 200 Hz and 500 Hz, reaction rate increased significantly with increase of magnetic field strength. When the magnetic field frequency was kept at 500 Hz, the reaction rate increased from 3.89 mu M/min to 8.11 mu M/min by increasing magnetic field strength from 1.3 to 14.2 Gs. The immobilized bi-enzyme system also showed good reusability and stability in the magnetic field (500 Hz, 14.2 Gs), that about 46% of original activity could be retained after 33 repeated uses, accounting for totally 34 days continuous operation. These results demonstrated the feasibility in intensifying molecular interactions among magnetic nanoparticle-supported multienzymes by using nano-magnetic stirrer for efficient multi-step transformations. (C) 2013 Elsevier B. V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | COLLOIDAL SILICA COATINGS ; MOBILITY ; ENZYMES ; PARTICLES ; AGENT ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325978300013 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13343]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Zheng, Muqing,Su, Zhiguo,Ji, Xiaoyuan,et al. Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles[J]. JOURNAL OF BIOTECHNOLOGY,2013,168(2):212-217. |
| APA | Zheng, Muqing,Su, Zhiguo,Ji, Xiaoyuan,Ma, Guanghui,Wang, Ping,&Zhang, Songping.(2013).Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles.JOURNAL OF BIOTECHNOLOGY,168(2),212-217. |
| MLA | Zheng, Muqing,et al."Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles".JOURNAL OF BIOTECHNOLOGY 168.2(2013):212-217. |
入库方式： OAI收割
来源：过程工程研究所
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