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Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli

文献类型：期刊论文

作者Wu, Dexi1; Li, Qiang2; Wang, Dan3; Dong, Yugang1
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
出版日期2013-08-01
卷号170期号:8页码:1942-1949
关键词Succinic acid Escherichia coli High solid concentration Enzymatic hydrolysis Fermentation
英文摘要Steam-exploded corn stalk biomass was used as the substrate for succinic acid production via lignocellulose enzymatic hydrolysis and fermentation. Succinic acid fermentation was investigated in Escherichia coli strains overexpressing cyanobacterium Anabaena sp. 7120 ecaA gene encoding carbonic anhydrase (CA). For the washed steam-exploded corn stalk at 30 % substrate concentration, i.e., 30 % water-insoluble solids (WIS), enzymatic hydrolysis yielded 97.5 g/l glucose solution and a cellulose conversion of 73.6 %, thus a high succinic acid level up to 38.6 g/l. With the unwashed steam-exploded corn stalk, though a cellulose conversion of 71.2 % was obtained in hydrolysis at 30 % solid concentration (27.9 % WIS), its hydrolysate did not ferment at all, and the hydrolysate of 25 % solid loading containing 3.8 g/l acetic acid and 168.2 mg/l furfural exerted a strong inhibition on succinic acid production.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]SIMULTANEOUS SACCHARIFICATION ; BIOREFINING STRATEGY ; ETHANOL ; GLUCOSE ; INHIBITION ; BIOMASS
收录类别SCI
语种英语
WOS记录号WOS:000322619100012
公开日期2015-05-27
源URL[http://ir.ipe.ac.cn/handle/122111/13358]  
专题过程工程研究所_研究所（批量导入）
作者单位1.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Cardiol, Guangzhou 510080, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Dexi,Li, Qiang,Wang, Dan,et al. Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(8):1942-1949.
APA Wu, Dexi,Li, Qiang,Wang, Dan,&Dong, Yugang.(2013).Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(8),1942-1949.
MLA Wu, Dexi,et al."Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.8(2013):1942-1949.

入库方式： OAI收割

来源：过程工程研究所

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