Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine
文献类型：期刊论文
| 作者 | Zhang, Jie1; Zhou, Xiaohua1; Wang, Dan1; Wang, Yingli1; Zhou, Xing2; Wang, Honghui1; Li, Qiang3; Tan, Shiyu1 |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2013-12-15 |
| 卷号 | 97期号:0页码:80-86 |
| 关键词 | Crosslinked chitosan microspheres Co-immobilized GOD/CAT GOD performance H2O2 Lysine |
| 英文摘要 | Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on a novel crosslinked chitosan resin modified by L-lysine. The immobilized system exhibited the best performance when the immobilization process was conducted at 4 degrees C for 3 h with GOD/CAT activity ratio of 1:18 and pH 7.0. Under the optimum pH 4.0 and temperature 40 degrees C, the enzyme system could achieve to the maximum conversion of glucose, which was 68.9%. Acidoresistance and thermotolerance of the enzymes were both strengthened. The Km of the co-immobilized enzyme system obtained in this study was 31.02 mM. The GOD enzyme immobilized in this system could maintain 94% of initial activity after the 12th operational cycle. 95.8% of initial activity was left after 50 weeks' storage at 4 degrees C. GOD achieved better performance in this new immobilized enzyme system due to its effective elimination of H2O2, which showed potential applications for various commercial uses. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | GLUCOSE-OXIDASE ; COVALENT IMMOBILIZATION ; ENZYME IMMOBILIZATION ; CALCIUM GLUCONATE ; MICROBIAL LIPASE ; CATALASE ; MEMBRANES ; OXIDATION ; KINETICS ; LINKING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327005000013 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13361]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China 2.Chongqing Acad Chinese Mat Med, TCM Pharmacochem Inst, Chongqing 400065, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jie,Zhou, Xiaohua,Wang, Dan,et al. Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,97(0):80-86. |
| APA | Zhang, Jie.,Zhou, Xiaohua.,Wang, Dan.,Wang, Yingli.,Zhou, Xing.,...&Tan, Shiyu.(2013).Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,97(0),80-86. |
| MLA | Zhang, Jie,et al."Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 97.0(2013):80-86. |
入库方式： OAI收割
来源：过程工程研究所
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