Immobilization of Papain in Biosilica Matrix and Its Catalytic Property
文献类型：期刊论文
| 作者 | Zhou Liya1; Wang Cui1; Jiang Yanjun1,2; Gao Jing1 |
| 刊名 | CHINESE JOURNAL OF CHEMICAL ENGINEERING
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 21期号:6页码:670-675 |
| 关键词 | papain silica matrix encapsulation biosilicification biomimetic |
| ISSN号 | 1004-9541 |
| 英文摘要 | A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | BIOMIMETICALLY SYNTHESIZED SILICA ; ENZYME ENCAPSULATION ; PROTEIN ; EFFICIENT ; ENTRAPMENT ; LYSOZYME ; SUPPORT ; TITANIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322510200011 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13374]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Liya,Wang Cui,Jiang Yanjun,et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,2013,21(6):670-675. |
| APA | Zhou Liya,Wang Cui,Jiang Yanjun,&Gao Jing.(2013).Immobilization of Papain in Biosilica Matrix and Its Catalytic Property.CHINESE JOURNAL OF CHEMICAL ENGINEERING,21(6),670-675. |
| MLA | Zhou Liya,et al."Immobilization of Papain in Biosilica Matrix and Its Catalytic Property".CHINESE JOURNAL OF CHEMICAL ENGINEERING 21.6(2013):670-675. |
入库方式： OAI收割
来源：过程工程研究所
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