Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction
文献类型：期刊论文
| 作者 | Deng, Fuli1,2,3; Liang, Xiangfeng1; Yang, Liangrong1; Liu, Qingfen1; Liu, Huizhou1,2 |
| 刊名 | PHYTOCHEMICAL ANALYSIS
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 24期号:4页码:381-385 |
| 关键词 | cloud-point extraction HPLC Genapol (R) X-080 mogroside V Siraitia grosvenorii |
| 英文摘要 | Introduction Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre-treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming. Objective To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii. Methods Mogroside V was extracted and preconcentrated by micelle-mediated cloud-point extraction with nonionic surfactant isotridecyl poly (ethylene glycol) ether (Genapol (R) X-080). The obtained solutions containing mogroside V were analysed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The chromatographic separation was performed on a C18-column using gradient elution with acetonitrile and water at 203nm. Results The cloud-point extraction yield was 80.7% while the pre-concentration factor was about 10.8. The limit of detection was 0.75 mu g/mL and the limit of quantification was 2 mu g/mL. The relative standard deviations for intra- and interday precisions of mogroside V were less than 8.68% and 5.78%, respectively, and the recoveries were between 85.1% and 103.6%. Conclusion The HPLC-UV method based on micelle-mediated cloud-point extraction for determination mogroside V in Siraitia grosvenorii was environmentally friendly, simple and sensitive. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; POLYCYCLIC AROMATIC-HYDROCARBONS ; CUCURBITANE GLYCOSIDES ; PRECONCENTRATION ; SEPARATION ; MEDICINE ; SYSTEMS ; FRUITS ; SERUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320558200013 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13390]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Fuli,Liang, Xiangfeng,Yang, Liangrong,et al. Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction[J]. PHYTOCHEMICAL ANALYSIS,2013,24(4):381-385. |
| APA | Deng, Fuli,Liang, Xiangfeng,Yang, Liangrong,Liu, Qingfen,&Liu, Huizhou.(2013).Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction.PHYTOCHEMICAL ANALYSIS,24(4),381-385. |
| MLA | Deng, Fuli,et al."Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction".PHYTOCHEMICAL ANALYSIS 24.4(2013):381-385. |
入库方式： OAI收割
来源：过程工程研究所
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