Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification
文献类型：期刊论文
| 作者 | Luo, Jian1; Zhou, Weiqing1; Su, Zhiguo1; Ma, Guanghui1; Gu, Tingyue2 |
| 刊名 | CHEMICAL ENGINEERING SCIENCE
 |
| 出版日期 | 2013-10-11 |
| 卷号 | 102期号:0页码:99-105 |
| 关键词 | Size exclusion chromatography Cored beads Modeling Protein purification Mass transfer |
| 英文摘要 | Size-exclusion chromatography (SEC) relies exclusively on intraparticle diffusion to separate solutes of different molecular sizes and shapes. Thus, its feed volume can only be a small fraction of the column volume. Much larger columns are required for SEC than other forms of liquid chromatography. Becasue of this, SEC often employs less expensive soft gels in large-scale applications to reduce costs. Excessive bed compression forces engineers to use pancake-shaped columns instead of more desirable slim columns during scale-up. Cored beads have impenetrable rigid cores that result in lower pressure drops and better pressure resistance. They also provide sharper peaks due to shortened radial distance for diffusion. Using a new general rate model for SEC with cored beads, this work demonstrated that cored beads performed better than fully-porous beads for myoglobin and ovalbumin separation through computer simulation. This theoretical work could encourge the research and product development of cored beads for large-scale SEC that has not been reported. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HEPATITIS-B-VIRUS ; PACKED-BEDS ; EXCHANGE CHROMATOGRAPHY ; PACKING MATERIALS ; MASS TRANSFER ; MEDIA ; MODEL ; SCALE ; FLOW |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325945500010 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13465]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Ohio Univ, Dept Chem & Biomol Engn, Athens, OH 45701 USA |
| 推荐引用方式 GB/T 7714 | Luo, Jian,Zhou, Weiqing,Su, Zhiguo,et al. Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification[J]. CHEMICAL ENGINEERING SCIENCE,2013,102(0):99-105. |
| APA | Luo, Jian,Zhou, Weiqing,Su, Zhiguo,Ma, Guanghui,&Gu, Tingyue.(2013).Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification.CHEMICAL ENGINEERING SCIENCE,102(0),99-105. |
| MLA | Luo, Jian,et al."Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification".CHEMICAL ENGINEERING SCIENCE 102.0(2013):99-105. |
入库方式： OAI收割
来源：过程工程研究所
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