A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production
文献类型：期刊论文
| 作者 | Mahmood, Iram1,3; Ahmad, Ishfaq2; Chen, Guo3; Liu Huizhou3 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2013-04-15 |
| 卷号 | 73期号:0页码:72-79 |
| 关键词 | Magnetic nanoparticles Gum arabic Lipase Esterification |
| 英文摘要 | Gum arabic coated magnetic Fe3O4 nanoparticles (GAMNP) were prepared by chemical co-precipitation method and their surface morphology, particle size and presence of polymer-coating was confirmed by various measurements, including transmission electron microscopy (TEM), X-ray diffraction (XRD), thermo gravimetric analysis (TGA), and Fourier transform infra red (FTIR) analysis. Magnetic particles were employed for their potential application as a support material for lipase immobilization. Glutaraldehyde was used as a coupling agent for efficient binding of lipase onto the magnetic carrier. For this purpose, the surface of a Candida rugosa lipase was initially coated with various surfactants, to stabilize enzyme in its open form, and then immobilized on to the support. This immobilized system was used as a biocatalyst for ethyl isovalerate, a flavor ester, production. The influence of various factors such as type of surfactant, optimum temperature and pH requirement, organic solvent used, amount of surfactant in coating lipase and effect of enzyme loadings on the esterification reaction were systematically studied. Different surfactants were used amongst which non-ionic surfactant performed better, showing about 80% esterification yield in 48 h as compared to cationic/anionic surfactants. Enhanced activity due to interfacial activation was observed for immobilized non-ionic surfactant-lipase complex. The immobilized surfactant coated lipase activity was retained after reusing seven times. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | CANDIDA-RUGOSA LIPASE ; INTERFACIAL ACTIVATION ; ORGANIC-SOLVENTS ; FLAVOR ESTERS ; ENZYMES ; PARTICLES ; HYDROLYSIS ; CATALYSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317453400010 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13524]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Natl Univ Sci & Technol, Sch Chem & Mat Engn, Islamabad 44000, Pakistan 2.Int Islamic Univ, Dept Math & Stat, Islamabad, Pakistan 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mahmood, Iram,Ahmad, Ishfaq,Chen, Guo,et al. A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production[J]. BIOCHEMICAL ENGINEERING JOURNAL,2013,73(0):72-79. |
| APA | Mahmood, Iram,Ahmad, Ishfaq,Chen, Guo,&Liu Huizhou.(2013).A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production.BIOCHEMICAL ENGINEERING JOURNAL,73(0),72-79. |
| MLA | Mahmood, Iram,et al."A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production".BIOCHEMICAL ENGINEERING JOURNAL 73.0(2013):72-79. |
入库方式： OAI收割
来源：过程工程研究所
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