Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties
文献类型：期刊论文
| 作者 | Zhou, Ying1; Pan, Siyi1; Wei, Xuetuan2; Wang, Lufeng1; Liu, Yanlong1 |
| 刊名 | BIORESOURCES
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:2页码:2605-2619 |
| 关键词 | Magnetic nanoparticles beta-glucosidase Immobilization Response surface methodology Characterization |
| 英文摘要 | This paper reports on a novel and efficient beta-glucosidase immobilization method using magnetic Fe3O4 nanoparticles as a carrier. Based on response surface methodology, the optimal immobilization conditions obtained were: glutaraldehyde (GA) concentration, 0.20%; enzyme concentration, 50.25 mu g/mL; cross-linking time, 2.21 h; and the maximum activity recovery reached 89.35%. The magnetic immobilized enzyme was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). FTIR revealed that beta-glucosidase was successfully immobilized on the magnetic nanoparticles. TEM showed that enzyme-magnetic nanoparticles possessed nano-scale size distribution. VSM confirmed that the enzyme-magnetic nanoparticles were superparamagnetic. The properties of the immobilized beta-glucosidase were improved, and the immobilized beta-glucosidase exhibited wider pH and temperature ranges of activation, higher accessibility of the substrate, better thermal stability, and better storage stability than that of the free enzyme. The enzyme-magnetic nanoparticles could be separated magnetically for easy reuse. Immobilization of beta-glucosidase onto the magnetic nanoparticles has the potential for industrial application. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Materials Science, Paper & Wood |
| 研究领域[WOS] | Materials Science |
| 关键词[WOS] | FE3O4 NANOPARTICLES ; ALPHA-AMYLASE ; BEADS ; CHITOSAN ; LIPASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320185500045 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13537]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ying,Pan, Siyi,Wei, Xuetuan,et al. Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties[J]. BIORESOURCES,2013,8(2):2605-2619. |
| APA | Zhou, Ying,Pan, Siyi,Wei, Xuetuan,Wang, Lufeng,&Liu, Yanlong.(2013).Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties.BIORESOURCES,8(2),2605-2619. |
| MLA | Zhou, Ying,et al."Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties".BIORESOURCES 8.2(2013):2605-2619. |
入库方式： OAI收割
来源：过程工程研究所
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