Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1
文献类型：期刊论文
| 作者 | Cao, Weifeng1,2; Qi, Benkun1; Zhao, Juan3; Qiao, Changsheng4; Su, Yi1; Wan, Yinhua1 |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 88期号:5页码:808-817 |
| 关键词 | -poly(malic acid) Aureobasidium pullulans dissolved oxygen concentration stirring speed |
| 英文摘要 | BACKGROUND: -poly(malic acid) (PMLA) can be used as a pro-drug or for a drug-delivery system. Effects of pH, dissolved oxygen concentration (DO) and stirring speed were investigated to improve PMLA production by A. pullulans ipe-1. RESULTS: The strain produced a high PMLA concentration when pH and DO remained at about 6.0 and above 70%, respectively, and the yeast-like cells were the main PMLA producers. To further promote PMLA production, the cultivation could be divided into three phases. In phase I, cell growth was accelerated by maintaining high DO (>70%) with a constant stirring speed of 800 rpm. In phase II, PMLA production was increased by controlling DO at 70% using the automatically controlled stirring speed. In phase III, PMLA production on per gram of glucose (Yp/s) was enhanced by keeping DO at 70%, and using a low stirring speed to decrease cell growth. Compared with batch cultures, a higher PMLA yield was obtained with this strategy, i.e. PMLA production and Yp/s increased by 15% and 18%, respectively. CONCLUSION: Control strategies for pH, DO and stirring speed provide a good reference for process development and optimization of PMLA production. (c) 2012 Society of Chemical Industry |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | EXOPOLYSACCHARIDE PRODUCTION ; PHYSARUM-POLYCEPHALUM ; POLY(BETA-MALIC ACID) ; MOLECULAR-WEIGHT ; IMPELLER SPEED ; DRUG-DELIVERY ; CULTURE ; GROWTH ; FERMENTATION ; AGITATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317674400009 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13591]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Res Ctr Modern Anal Technol, Tianjin 300457, Peoples R China 4.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Qi, Benkun,Zhao, Juan,et al. Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2013,88(5):808-817. |
| APA | Cao, Weifeng,Qi, Benkun,Zhao, Juan,Qiao, Changsheng,Su, Yi,&Wan, Yinhua.(2013).Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,88(5),808-817. |
| MLA | Cao, Weifeng,et al."Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 88.5(2013):808-817. |
入库方式： OAI收割
来源：过程工程研究所
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