Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins
文献类型：期刊论文
| 作者 | Li, Wensong1,2; Yang, Liangrong1; Zhou, Huacong1,2; Li, Xiaopei1,2; Wang, Fuchun1,2; Yang, Xingfu1; Liu, Huizhou1 |
| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
 |
| 出版日期 | 2013-11-20 |
| 卷号 | 52期号:46页码:16314-16320 |
| 英文摘要 | In this study, gas-assisted superparamagnetic extraction (GASE) with potential large-scale application was proposed for selective separation of multicomponent proteins. Magnetic poly(glycidyl methacrylate)-iminodiacetic acid Zn2+ microspheres (MPMs) for selective separation of bovine serum albumin (BSA) and bovine hemoglobin (BHb) are selected as a model. The feasibility of flotation for concentrating BHb-loaded MPMs from dilute mixed proteins solution was proved. The well-concentrated solution of BHb-loaded MPMs could be achieved by simple adjustment of pH values and ionic strength in low regions, without additive detergent. Impurity BSA had the effects of improving the flotability of BHb-loaded MPMs, acting as foaming agent, but decreasing the enrichment ratio in the GASE process. The flotation conditions and the selective adsorption ones showed strong consistency. Under the optimal conditions, the enrichment ratio of 39 and recovery percentage of 98% was obtained within 1.3 min. Furthermore, the enrichment ratio could be further improved by added flotation steps. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; PURIFICATION ; ADSORPTION ; PARTICLES ; FLOTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327413500024 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13597]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wensong,Yang, Liangrong,Zhou, Huacong,et al. Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2013,52(46):16314-16320. |
| APA | Li, Wensong.,Yang, Liangrong.,Zhou, Huacong.,Li, Xiaopei.,Wang, Fuchun.,...&Liu, Huizhou.(2013).Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,52(46),16314-16320. |
| MLA | Li, Wensong,et al."Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 52.46(2013):16314-16320. |
入库方式： OAI收割
来源：过程工程研究所
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