Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay
文献类型：期刊论文
| 作者 | Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xia, Tingting1; Xiong, Wubin1; Wang, Ning3; Guo, Chen3 |
| 刊名 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 405期号:0页码:291-295 |
| 关键词 | Magnetic nanoparticle Peroxidase-like activity Immunoassay |
| 英文摘要 | Amino-functionalized Fe3O4 magnetic nanoparticles with high peroxidase-like activity (MNPs-NH2) are reported in this work. The peroxidase-like activity is approximately the same as naked Fe3O4 magnetic nanoparticles (MNPs). MNPs-NH2 displayed much better thermal stability and pH tolerance than horse-radish peroxidase (HRP). The optimal pH and temperature are approximately pH 4 and 40 degrees C, respectively. We showed that the MNPs-NH2 were successfully applied in a double-antibody sandwich "enzyme"-linked immunosorbent assay to replace of HRP, in which MNPs-NH2 were not only separation carriers, but also detection indicator. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | FERROMAGNETIC NANOPARTICLES ; PARTICLES ; DELIVERY ; PROGRESS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321598400039 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13598]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2013,405(0):291-295. |
| APA | Yang, Mingzhu.,Guan, Yueping.,Yang, Yu.,Xia, Tingting.,Xiong, Wubin.,...&Guo, Chen.(2013).Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay.JOURNAL OF COLLOID AND INTERFACE SCIENCE,405(0),291-295. |
| MLA | Yang, Mingzhu,et al."Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay".JOURNAL OF COLLOID AND INTERFACE SCIENCE 405.0(2013):291-295. |
入库方式： OAI收割
来源：过程工程研究所
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