Preparation of Ultrathin, Robust Protein Microcapsules through Template-Mediated Interfacial Reaction between Amine and Catechol Groups
文献类型：期刊论文
| 作者 | Wang, Xiaoli1,3; Shi, Jiafu1,3; Jiang, Zhongyi1,2,3; Li, Zheng1; Zhang, Wenyan1,3; Song, Xiaokai1,3; Ai, Qinghong1,3; Wu, Hong1,3 |
| 刊名 | BIOMACROMOLECULES
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 14期号:11页码:3861-3869 |
| 英文摘要 | A novel approach to the synthesis of protein microcapsules is developed through template-mediated interfacial reaction. Protein-doped CaCO3 templates are first synthetized via coprecipitation and then coated with a catechol-containing alginate (AlgDA) layer. Afterward, the templates are exposed to ethylenediamine tetraacetic acid disodium (EDTA) solution to dissolve CaCO3. During CaCO3 dissolution, the generated CO2 gas pushes protein molecules moving to the AlgDA layer, and thereby Michael addition and Schiff base reactions proceed, forming the shell of protein microcapsules. Three kinds of proteins, namely, bovine serum albumin, catalase, and protamine sulfate, are utilized. The shell thickness of microcapsule varies from 25 to 82 nm as the doping amount of protein increased from 2 to 6 mg per 66 mg CaCO3. The protein microcapsules have a robust but flexible shell and can be reversibly deformed upon exposure to osmotic pressure. The bioactivity of protein microcapsules is demonstrated through enzymatic catalysis experiments. The protein microcapsules remain about 80% enzymatic activity of the equivalent free protein. Hopefully, our approach could be extended to many other applications such as drug/gene delivery, tissue scaffolds, and catalysis due to the universality of Michael reaction and Schiff base reactions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 关键词[WOS] | HUMAN SERUM-ALBUMIN ; CALCIUM-CARBONATE ; DRUG-DELIVERY ; ADDITION-REACTIONS ; IMMOBILIZATION ; CAPSULES ; MINERALIZATION ; ENCAPSULATION ; POLYDOPAMINE ; BIOMOLECULES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326955900007 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13608]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Chem Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Synerget Innovat Ctr Chem Sci & Engn, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaoli,Shi, Jiafu,Jiang, Zhongyi,et al. Preparation of Ultrathin, Robust Protein Microcapsules through Template-Mediated Interfacial Reaction between Amine and Catechol Groups[J]. BIOMACROMOLECULES,2013,14(11):3861-3869. |
| APA | Wang, Xiaoli.,Shi, Jiafu.,Jiang, Zhongyi.,Li, Zheng.,Zhang, Wenyan.,...&Wu, Hong.(2013).Preparation of Ultrathin, Robust Protein Microcapsules through Template-Mediated Interfacial Reaction between Amine and Catechol Groups.BIOMACROMOLECULES,14(11),3861-3869. |
| MLA | Wang, Xiaoli,et al."Preparation of Ultrathin, Robust Protein Microcapsules through Template-Mediated Interfacial Reaction between Amine and Catechol Groups".BIOMACROMOLECULES 14.11(2013):3861-3869. |
入库方式： OAI收割
来源：过程工程研究所
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