Low-temperature preincubation enhances survival and regeneration of cryopreserved Saussurea involucrata callus
文献类型：期刊论文
| 作者 | Guo, Bin; Stiles, Amanda R.; Liu, Chun-Zhao |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 49期号:3页码:320-325 |
| 关键词 | Cryopreservation Low-temperature preincubation Saussurea involucrata Vitrification |
| 英文摘要 | Saussurea involucrata Kar. et Kir. is one of the most well-known Chinese medicinal plants, and it is utilized for a variety of medical conditions. Due to the overexploitation of this endangered species, it is crucial to develop methods for both conservation and propagation. To address this issue, we have developed and optimized a simple and effective vitrification process for the cryopreservation of S. involucrata callus tissue. The optimized method consisted of a 3-d incubation period on medium containing 0.3 M sucrose, transfer to a plant vitrification solution (PVS2) containing 30% (v/v) glycerol, 15% (v/v) ethylene glycol, 15% (v/v) dimethylsulfoxide, and 0.4 M sucrose first at 60% PVS2 for 40 min, then at 100% PVS2 for 60 min, followed by immediate immersion and storage in liquid nitrogen. To thaw the tissue, tissues were rewarmed at 40A degrees C for 2 min. This method resulted in a survival rate of approximately 56% and a regrowth rate of approximately 40%. Survival and regrowth were significantly improved by the addition of a low-temperature preincubation step. Incubating the calli at 4A degrees C for 12 d prior to initiating the optimized cryopreservation protocol increased the survival rate of the tissue to 75%, increased the regrowth rate to 60%, and more than doubled the number of regenerated shoots per explant. Following cryopreservation, greater than 90% of the regenerated shoots formed complete plantlets, and 81% of the regenerated plantlets survived and grew vigorously under greenhouse conditions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 关键词[WOS] | IN-VITRO ; VITRIFICATION SOLUTIONS ; COLD-ACCLIMATION ; SHOOT-TIPS ; PLANTS ; CONSERVATION ; CULTURES ; EMBRYOS ; INJURY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319080400011 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13615]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Bin,Stiles, Amanda R.,Liu, Chun-Zhao. Low-temperature preincubation enhances survival and regeneration of cryopreserved Saussurea involucrata callus[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2013,49(3):320-325. |
| APA | Guo, Bin,Stiles, Amanda R.,&Liu, Chun-Zhao.(2013).Low-temperature preincubation enhances survival and regeneration of cryopreserved Saussurea involucrata callus.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,49(3),320-325. |
| MLA | Guo, Bin,et al."Low-temperature preincubation enhances survival and regeneration of cryopreserved Saussurea involucrata callus".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 49.3(2013):320-325. |
入库方式： OAI收割
来源：过程工程研究所
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