Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium
文献类型：期刊论文
| 作者 | Dai, Fengying1,2; Liu, Yuan1; Wang, Wei1; Liu, Wenguang1 |
| 刊名 | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 24期号:3页码:330-343 |
| 关键词 | 2-(dimethylamino) ethyl methacrylate sulfobetaine gene delivery salt stability |
| 英文摘要 | Cationic polyplexes would aggregate immediately after intravenous injection due to the plasma proteins and high ionic strength. A cationic polyplexes with long-term and salt stability was very important for a systemic gene therapy. In this research, a polysulfobetaine-b-polycation diblock copolymer composed of cationic block of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and zwtterionic block of poly(propylsulfonate dimethylammonium ethylmethacrylate) (PSPE) was conveniently synthesized by atom transfer radical polymerization method to obtain a cationic polymers with long-term and salt stability. The results of agarose gel electrophoresis and transmission electron microscope indicated that copolymerization of PSPE did not compromise the DNA condensation ability of PDMAEMA, meanwhile exhibiting lower cytotoxicity. The effect of salt on the absorbance and particle size of PDMAEMA(100)/DNA and PDMAEMA(100)-PSPEy/DNA complexes was investigated, which showed that PSPE block could increase the resistance of polyplexes against salt-induced aggregation owing to the antielectrolyte effect. In comparison with PDMAEMA homopolymer, PDMAEMA(100)-PSPEy retained more stable gene transfection in a certain range of salt concentration. The expression of red fluorescence protein (RFP) was evaluated by small animal in vivo fluorescence imaging system and the results showed that the expression of RFP was much higher in the mice injected with PDMAEMA(100)-PSPE(20/)pDNA-RFP than with PDMAEMA/pDNA-RFP. Both in vitro and in vivo results suggested that PDMAEMA-PSPE diblock copolymer may be potentially used as a vector for systemic gene therapy. |
| WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials ; Polymer Science |
| 研究领域[WOS] | Engineering ; Materials Science ; Polymer Science |
| 关键词[WOS] | SELF-ASSEMBLED MONOLAYERS ; DNA COMPLEXES ; PROTEIN ADSORPTION ; EXTENDED CIRCULATION ; PHYSICAL STABILITY ; MOLECULAR-WEIGHT ; PLASMID DNA ; IN-VIVO ; DELIVERY ; PEGYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316011600007 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13642]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Tianjin Univ, Sch Mat Sci & Engn, Tianjin Key Lab Composite & Funct Mat, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Fengying,Liu, Yuan,Wang, Wei,et al. Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium[J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,2013,24(3):330-343. |
| APA | Dai, Fengying,Liu, Yuan,Wang, Wei,&Liu, Wenguang.(2013).Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium.JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,24(3),330-343. |
| MLA | Dai, Fengying,et al."Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium".JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 24.3(2013):330-343. |
入库方式： OAI收割
来源：过程工程研究所
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