A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin
文献类型：期刊论文
| 作者 | Cui, Jian Dong1,2,3,4; Sun, Li Mei1; Li, Lian Lian1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 170期号:8页码:1827-1837 |
| 关键词 | Phenylalanine ammonia lyase Rhodotorula glutinis Cross-linked enzyme aggregates Co-aggregation Biocatalysts |
| 英文摘要 | Cross-linked enzyme aggregates (CLEAs) have been recently proposed as an alternative to conventional immobilization methods on solid carriers. However, the low cross-linking efficiency causes the major activity loss and instability in the conventional protocol for CLEA preparation. Herein, the effects of bovine serum albumin and starch addition on the cross-linking efficiency of CLEAs of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis were evaluated. A co-aggregation strategy was developed to improve cross-linking efficiency by adding starch and bovine serum albumin (BSA). CLEAs of PAL prepared in the presence of BSA and starch (PSB-CLEAs) retained 36 % activity, whereas CLEAs prepared without BSA and starch (PAL-CLEAs) retained only 8 % activity of the starting enzyme preparation. Compared with PAL-CLEAs, the thermal stability of PSB-CLEAs has improved considerably, maintaining 30 % residual activity after 4 h of incubation at 70 A degrees C, whereas the PAL-CLEAs have only 13 % residual activity. PSB-CLEAs also exhibited the expected increased stability of PAL against hydrophilic organic solvents, superior operability, and higher storage stability. The proposed technique of preparing CLEAs using co-aggregation with starch and BSA would rank among the potential strategies for efficiently preparing robust and highly stable enzyme aggregates. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | RHODOTORULA-GLUTINIS ; ENZYME ; BIOCATALYSTS ; PURIFICATION ; POLYMERS ; ACYLASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322619100003 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13652]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Hebei Univ Sci & Technol, Res Ctr Fermentat Engn Hebei, Coll Biosci & Bioengn, Shijiazhuang 050000, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China 4.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Jian Dong,Sun, Li Mei,Li, Lian Lian. A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(8):1827-1837. |
| APA | Cui, Jian Dong,Sun, Li Mei,&Li, Lian Lian.(2013).A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(8),1827-1837. |
| MLA | Cui, Jian Dong,et al."A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.8(2013):1827-1837. |
入库方式： OAI收割
来源：过程工程研究所
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