Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice
文献类型：期刊论文
| 作者 | Sun, Ying1,2,3; Liu, Ling2; Jiang, Lian-Zhou1; Zhang, Gui-Feng4; Li, Guo-Ming2; Wu, Na2 |
| 刊名 | EUROPEAN FOOD RESEARCH AND TECHNOLOGY
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 237期号:2页码:149-157 |
| 关键词 | Protease inhibitors Potato fruit juice Isolation Chemical composition Anti-obesity effects |
| 英文摘要 | In this study, potato protease inhibitors (PPI) were obtained from industrial potato fruit juice (PFJ) using membrane separation method and ion exchange chromatography. The molecular weight distribution, families, structural characterization, and in vitro anti-proliferative activity of PPI were evaluated in this paper. The molecular weight distribution of PPI was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS). The inhibitor families were identified using high-performance liquid chromatography/mass spectrometry. The structures of the isolated PPI were studied by circular dichroism and fluorescence spectroscopy. Anti-proliferative activity of PPI on 3T3-L1 preadipocytes was investigated by MTT method. The results suggest that ultrafiltration (UF) was more suitable for the isolation of crude proteins from PFJ than nanofiltration. The PPI purified from UF freeze-dried powder were mainly focused on proteins in the region of relative molecular masses (Mr) 20 x 10(3) to 22 x 10(3) with a few low molecular weight proteins, containing protease inhibitor, protease inhibitor II, aspartic protease inhibitor, and other serine protease inhibitor families. The major conformation of PPI was beta-structure, and the tryptophans were mainly located in hydrophobic environments. Furthermore, the structure changed significantly when the temperature reached 80 A degrees C. Moreover, PPI were able to inhibit the proliferation of 3T3-L1 preadipocytes with a 50 % inhibitory concentration of 343.51 mu g/mL. Therefore, PPI may have a potential implication in anti-obesity foods in the future. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Food Science & Technology |
| 研究领域[WOS] | Food Science & Technology |
| 关键词[WOS] | EMULSIFYING PROPERTIES ; ADIPOSE-TISSUE ; PROTEINS ; SOLUBILITY ; RECOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322260200007 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13663]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Northeast Agr Univ, Coll Food Sci, Harbin 150030, Heilongjiang, Peoples R China 2.China Natl Res Inst Food & Fermentat Ind, Beijing 100027, Peoples R China 3.Qiqihar Univ, Coll Food & Biol Engn, Qiqihar 161006, Heilongjiang, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Ying,Liu, Ling,Jiang, Lian-Zhou,et al. Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2013,237(2):149-157. |
| APA | Sun, Ying,Liu, Ling,Jiang, Lian-Zhou,Zhang, Gui-Feng,Li, Guo-Ming,&Wu, Na.(2013).Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,237(2),149-157. |
| MLA | Sun, Ying,et al."Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 237.2(2013):149-157. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。