中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli

文献类型:期刊论文

作者Wang, Ziqiang1,2; Wang, Yunshan1; Su, Zhiguo1
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2013-03-01
卷号97期号:6页码:2433-2441
关键词Cis-epoxysuccinic acid hydrolase Nocardia tartaricans Prokaryotic expression Enantioselectivity
英文摘要A highly enantioselective cis-epoxysuccinic acid hydrolase from Nocardia tartaricans was purified to electrophoretic homogeneity. The enzyme was purified 184-fold with a yield of 18.8 %. The purified cis-epoxysuccinic acid hydrolase had a monomeric molecular weight of 28 kDa, and its optimum conditions were 37 A degrees C and pH 7-9. With sodium cis-epoxysuccinate as the substrate, Michaelis-Menten enzyme kinetics analysis gave a Km value of 35.71 mM and a Vmax of 2.65 mM min(-1). The enzyme was activated by Ni2+ and Al3+, while strongly inhibited by Fe3+, Fe2+, Cu2+, and Ag+. The cis-epoxysuccinic acid hydrolase gene was cloned, and its open reading frame sequence predicted a protein composed of 253 amino acids. A pET11a expression plasmid carrying the gene under the control of the T7 promoter was introduced into Escherichia coli, and the cis-epoxysuccinic acid hydrolase gene was successfully expressed in the recombinant strains.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]AGROBACTERIUM-RADIOBACTER AD1 ; MICROBIAL EPOXIDE HYDROLASES ; ASPERGILLUS-NIGER M200 ; DIRECTED EVOLUTION ; ENANTIOSELECTIVITY ; HYDROLYSIS ; MECHANISM ; CLONING ; ENZYME ; CELLS
收录类别SCI
语种英语
WOS记录号WOS:000315650100013
公开日期2015-05-27
源URL[http://ir.ipe.ac.cn/handle/122111/13741]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Wang, Ziqiang,Wang, Yunshan,Su, Zhiguo. Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2433-2441.
APA Wang, Ziqiang,Wang, Yunshan,&Su, Zhiguo.(2013).Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2433-2441.
MLA Wang, Ziqiang,et al."Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2433-2441.

入库方式: OAI收割

来源:过程工程研究所

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