Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass
文献类型：期刊论文
| 作者 | Zheng, Yingfu1,2; Zhang, Songping1; Miao, Shida1; Su, Zhiguo1; Wang, Ping3,4 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2013-07-10 |
| 卷号 | 166期号:3页码:135-143 |
| 关键词 | Cellulase Lignin Enzyme adsorption Steric hindrance Biomass recalcitrance Cellulose hydrolysis |
| 英文摘要 | Unproductive enzyme adsorption is an important factor in addition to steric hindrance of lignin that limits the enzymatic hydrolysis of lignocellulosic biomass. While both are important factors, enzymatic hydrolysis of pretreated biomass is most likely conducted in the presence of certain amount of lignin residues that may not necessarily present accessibility hindrance, but can competitively absorb the enzyme. This paper presents a study with purified lignin samples to elucidate the role of unproductive enzyme adsorption. It appeared that lignin adsorbed cellulase quickly at 4 degrees C with adsorption equilibrium reached within 1 h, similar to that observed for crystalline cellulose. Increasing temperature to 50 degrees C (typical hydrolytic reaction condition) facilitated the rate of cellulase adsorption on cellulose with a peak of adsorption reached at 0.25 h; however, adsorption on lignin was surprisingly slower and took over 12 h to reach equilibrium, which was accompanied with a 10-fold increase in adsorption capacity. Despite the high adsorption capacity of lignin (which is comparable to that of cellulose) at 50 degrees C, the presence of added lignin imposed only minimal impact on the enzyme apparent activity, most likely due to the slow adsorption kinetics of lignin. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | STEAM PRETREATED SOFTWOOD ; TRICHODERMA-REESEI ; MICROCRYSTALLINE CELLULOSE ; CORN STOVER ; LODGEPOLE PINE ; ENZYMES ; BINDING ; HEMICELLULOSE ; TECHNOLOGIES ; INHIBITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321120200009 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13744]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Zheng, Yingfu,Zhang, Songping,Miao, Shida,et al. Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass[J]. JOURNAL OF BIOTECHNOLOGY,2013,166(3):135-143. |
| APA | Zheng, Yingfu,Zhang, Songping,Miao, Shida,Su, Zhiguo,&Wang, Ping.(2013).Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass.JOURNAL OF BIOTECHNOLOGY,166(3),135-143. |
| MLA | Zheng, Yingfu,et al."Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass".JOURNAL OF BIOTECHNOLOGY 166.3(2013):135-143. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。