Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed
文献类型：期刊论文
| 作者 | Wang, Feng; Guo, Chen; Liu, Chun-Zhao |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 171期号:8页码:2165-2175 |
| 关键词 | Magnetically stabilized fluidized bed Magnetic mesoporous nanoparticles Axial liquid dispersion Laccase purification |
| 英文摘要 | A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu2+ particles in the MSFB maintained a stable bed expansion of two to threefold at a flow rate of 120-180 cm/h. At the optimal magnetic field intensity of 120 Gs, both the maximal Bodenstein number and the smallest axial dispersion coefficient were achieved, which resulted in a stable fluidization stage. The dynamic binding capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with MMSNPs-Cu2+ achieved efficient laccase purification from the fermentation broth with 62.4-fold purification of laccase and 108.9 % activity yield. These results provided an excellent platform for the application of these magnetic mesoporous nanoparticles integrated with the MSFB in developing novel protein purification process. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SILICA NANOPARTICLES ; TRAMETES-VERSICOLOR ; PROTEIN ADSORPTION ; AXIAL-DISPERSION ; SEPARATION ; PARTICLES ; BEADS ; CHROMATOGRAPHY ; MICROSPHERES ; PERFORMANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327494800021 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13745]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Feng,Guo, Chen,Liu, Chun-Zhao. Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,171(8):2165-2175. |
| APA | Wang, Feng,Guo, Chen,&Liu, Chun-Zhao.(2013).Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,171(8),2165-2175. |
| MLA | Wang, Feng,et al."Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 171.8(2013):2165-2175. |
入库方式： OAI收割
来源：过程工程研究所
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