中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed

文献类型:期刊论文

作者Wang, Feng; Guo, Chen; Liu, Chun-Zhao
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
出版日期2013-12-01
卷号171期号:8页码:2165-2175
关键词Magnetically stabilized fluidized bed Magnetic mesoporous nanoparticles Axial liquid dispersion Laccase purification
英文摘要A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu2+ particles in the MSFB maintained a stable bed expansion of two to threefold at a flow rate of 120-180 cm/h. At the optimal magnetic field intensity of 120 Gs, both the maximal Bodenstein number and the smallest axial dispersion coefficient were achieved, which resulted in a stable fluidization stage. The dynamic binding capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with MMSNPs-Cu2+ achieved efficient laccase purification from the fermentation broth with 62.4-fold purification of laccase and 108.9 % activity yield. These results provided an excellent platform for the application of these magnetic mesoporous nanoparticles integrated with the MSFB in developing novel protein purification process.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]SILICA NANOPARTICLES ; TRAMETES-VERSICOLOR ; PROTEIN ADSORPTION ; AXIAL-DISPERSION ; SEPARATION ; PARTICLES ; BEADS ; CHROMATOGRAPHY ; MICROSPHERES ; PERFORMANCE
收录类别SCI
语种英语
WOS记录号WOS:000327494800021
公开日期2015-05-27
源URL[http://ir.ipe.ac.cn/handle/122111/13745]  
专题过程工程研究所_研究所(批量导入)
作者单位Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Wang, Feng,Guo, Chen,Liu, Chun-Zhao. Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,171(8):2165-2175.
APA Wang, Feng,Guo, Chen,&Liu, Chun-Zhao.(2013).Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,171(8),2165-2175.
MLA Wang, Feng,et al."Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 171.8(2013):2165-2175.

入库方式: OAI收割

来源:过程工程研究所

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