Gas-Assisted Superparamagnetic Extraction for Potential Large-Scale Separation of Proteins
文献类型：期刊论文
| 作者 | Li, Wensong1,2; Yang, Liangrong1; Wang, Fuchun1,2; Zhou, Huacong1,2; Xing, Huifang1 ; Li, Xiaopei1,2; Liu, Huizhou1
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| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
 |
| 出版日期 | 2013-03-20 |
| 卷号 | 52期号:11页码:4290-4296 |
| 英文摘要 | In this study, gas-assisted superparamagnetic extraction (GASE), a novel technology that combined superparamagnetic extraction technology with flotation technology, was proposed for potential large-scale separation of proteins. GASE includes adsorption for trapping proteins, and flotation and high gradient magnetic separator (HGMS) for recovering proteins-loaded magnetic particles from the dilute biosuspension. Citrate-modified superparamagnetic nanoparticles (CMNs) and bovine serum albumin (BSA) were used as a model. The feasibility of flotation for these particles enrichment was demonstrated. The results indicated that the BSA-loaded CMNs could be well concentrated in either foaming flotation or nonfoaming flotation by simple adjustment of the initial solution pH, without extra detergents and operations. The flotation conditions were consistent with the adsorption ones, and no proteins were desorbed from CMNs in the GASE process. Under the optimal conditions, the enrichment ratio and recovery percentage reached 31 and 99% in 1.5 min, respectively. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | MAGNETIC NANOPARTICLES ; ADSORPTION ; PURIFICATION ; FLOTATION ; PARTICLES ; ALBUMIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316594200033 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13749]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wensong,Yang, Liangrong,Wang, Fuchun,et al. Gas-Assisted Superparamagnetic Extraction for Potential Large-Scale Separation of Proteins[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2013,52(11):4290-4296. |
| APA | Li, Wensong.,Yang, Liangrong.,Wang, Fuchun.,Zhou, Huacong.,Xing, Huifang.,...&Liu, Huizhou.(2013).Gas-Assisted Superparamagnetic Extraction for Potential Large-Scale Separation of Proteins.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,52(11),4290-4296. |
| MLA | Li, Wensong,et al."Gas-Assisted Superparamagnetic Extraction for Potential Large-Scale Separation of Proteins".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 52.11(2013):4290-4296. |
入库方式： OAI收割
来源：过程工程研究所
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