Determination of leakage from antibody adsorbent: composition analysis and pH effect
文献类型：期刊论文
| 作者 | Zhao, Lan; Liu, Yongdong; Wang, Yinjue; Huang, Yongdong; Li, Xiunan; Li, Yan; Ma, Guanghui; Su, Zhiguo |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 27期号:9页码:1089-1091 |
| 关键词 | immunoadsorbent human immunoglobulin G ligand leakage matrix leakage pH-dependent |
| 英文摘要 | To study the leakage at different solution pH values, IgG Sepharose 6FF (R), a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the kinetics and the amount of ligand leakage. Cross-linking of the adsorbent matrix could reduce both matrix leakage and antibody leakage at pH 3.0, but its effect was limited at pH 11.0 for ligand leakage. Copyright (C) 2013 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 关键词[WOS] | FAST-FLOW ; CHROMATOGRAPHY ; IMMUNOADSORBENTS ; STABILITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322717100001 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13762]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lan,Liu, Yongdong,Wang, Yinjue,et al. Determination of leakage from antibody adsorbent: composition analysis and pH effect[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(9):1089-1091. |
| APA | Zhao, Lan.,Liu, Yongdong.,Wang, Yinjue.,Huang, Yongdong.,Li, Xiunan.,...&Su, Zhiguo.(2013).Determination of leakage from antibody adsorbent: composition analysis and pH effect.BIOMEDICAL CHROMATOGRAPHY,27(9),1089-1091. |
| MLA | Zhao, Lan,et al."Determination of leakage from antibody adsorbent: composition analysis and pH effect".BIOMEDICAL CHROMATOGRAPHY 27.9(2013):1089-1091. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。