Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization
文献类型：期刊论文
| 作者 | Liu, Ying1; Guo, Chen2; Sun, Xi-Tong2; Liu, Chun-Zhao1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 142期号:0页码:415-419 |
| 关键词 | Yarrowia lipolytica lipase Bioimprinting Immobilization Resolution 2-Octanol |
| 英文摘要 | Yarrowia lipolytica lipase (YLL) demonstrated an (R)-enantiopreference for efficient resolution of (R,S)-2-octanol. The activity, enantioselectivity, the ratio of substrate to enzyme, acetaldehyde tolerance, and operational stability of YLL were improved by an integrated strategy of interfacial activation, bioimprinting, and immobilization. In comparison with the control, both the enzymatic activity and enantioselectivity increased by a factor of 8.85 and 2.75 by the integrated strategy, respectively. Fifty-one percentage of conversion with 220 of enantioselectivity was obtained using the immobilized YLL prepared by the integrated strategy at a ratio of 104 of substrate to enzyme loaded. The immobilized YLL retained 97% of its initial activity without a decrease in enantioselectivity after 10 successive reuse cycles. Together these results will result in a promising strategy with the YYL for efficient resolution of (R,S)-2-octanol in practice. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | POLYUNSATURATED FATTY-ACIDS ; MACROPOROUS RESIN ; ACETALDEHYDE ; ADSORPTION ; STABILIZATION ; PROTEINS ; ENZYMES ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322292800055 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13768]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Guo, Chen,Sun, Xi-Tong,et al. Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization[J]. BIORESOURCE TECHNOLOGY,2013,142(0):415-419. |
| APA | Liu, Ying,Guo, Chen,Sun, Xi-Tong,&Liu, Chun-Zhao.(2013).Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization.BIORESOURCE TECHNOLOGY,142(0),415-419. |
| MLA | Liu, Ying,et al."Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization".BIORESOURCE TECHNOLOGY 142.0(2013):415-419. |
入库方式： OAI收割
来源：过程工程研究所
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