Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test
文献类型：期刊论文
| 作者 | Xu, Jiandong1; Lv, Xuefei2; Wei, Yuping1; Zhang, Liang1,3; Li, Rui2; Deng, Yulin2; Xu, Xia1 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 212期号:JUN页码:472-480 |
| 关键词 | Dispoable microPCR chip NOA81 Portable and programmable PCR device Air bubble resistant |
| ISSN号 | 0925-4005 |
| 通讯作者 | Deng, YL (reprint author), 1 Beiertiao, Beijing 100190, Peoples R China. |
| 英文摘要 | Due to the difficulty in sample introduction, the presence of air bubbles and the complicated fabrication with high cost, there are no commercial products of microfluidic polymerase chain reaction (microPCR) on the market yet. In this study, an air bubble resistant and disposable microPCR chip was developed. A simple fabrication method using Norland Optical Adhesive 81 (NOA81) as the substrates was employed to prepare the microPCR chip. The temperature of the microPCR chip was controlled using a self-developed portable and programmable PCR apparatus. The simulation results indicate that the developed microPCR chip has good performance in temperature stability even in the presence of air bubbles. The DNA was successfully amplified without adding bovine serum albumin, implicating that the developed microPCR chip has low adsorption of DNA polymerase. The good performance of microPCR chip attributed to the property of N0A81, the fabrication method and the irreversible sealing. The portable and programmable PCR device combined with the disposable microPCR chip is suitable for the amplification of both short and long DNA fragments and Short Tandem Repeats (STRs). It offers a practical approach for portable forensic test, combining the cost benefits of small volume reagent with the disposable polymer microPCR chip. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 学科主题 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 类目[WOS] | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 关键词[WOS] | REAL-TIME PCR ; MICROFLUIDIC DEVICES ; DNA AMPLIFICATION ; MICROCHIP ; SYSTEM ; LAB |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351017700061 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/13773]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jiandong,Lv, Xuefei,Wei, Yuping,et al. Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test[J]. SENSORS AND ACTUATORS B-CHEMICAL,2015,212(JUN):472-480. |
| APA | Xu, Jiandong.,Lv, Xuefei.,Wei, Yuping.,Zhang, Liang.,Li, Rui.,...&Xu, Xia.(2015).Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test.SENSORS AND ACTUATORS B-CHEMICAL,212(JUN),472-480. |
| MLA | Xu, Jiandong,et al."Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test".SENSORS AND ACTUATORS B-CHEMICAL 212.JUN(2015):472-480. |
入库方式： OAI收割
来源：过程工程研究所
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