中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 过程工程研究所
  3. 中国科学院过程工程研究所
  4. 研究所（批量导入）
Characterization of a new endo-type alginate lyase from Vibrio sp W13

文献类型：期刊论文

作者Zhu, Benwei1,2; Tan, Haidong1; Qin, Yuqi3; Xu, Qingsong1; Du, Yuguang1,4; Yin, Heng1
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2015-04-01
卷号75期号:APR页码:330-337
关键词Alginate lyase Polysaccharide lyase family 7 Vibrio sp.
ISSN号0141-8130
通讯作者Du, YG (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.
英文摘要A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 degrees C Interestingly, Algb possessed broader substrate specificity. It showed activities toward both polyM (poly beta-D-mannuronate) and polyG (poly alpha-L-guluronate). Furthermore, K-m values of Algb toward alginate (0.67 mg/ml) and polyMG (0.50 mg/ml) are lower than those toward polyG (1.04 mg/ml) and polyM (6.90 mg/ml). The TLC and ESI-MS analysis suggested that Algb mainly released oligosaccharides with DP of 2-5 from the four kinds of substrates in an endolytic manner. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DP. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Biochemistry & Molecular Biology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]OLIGOALGINATE LYASE ; SEQUENCE-ANALYSIS ; SACCHAROPHAGUS DEGRADANS ; CYSTIC-FIBROSIS ; RAW264.7 CELLS ; CLONING ; PURIFICATION ; OLIGOSACCHARIDES ; POLYSACCHARIDE ; DEPOLYMERIZATION
收录类别SCI
语种英语
WOS记录号WOS:000352332700044
源URL[http://ir.ipe.ac.cn/handle/122111/13785]  
专题过程工程研究所_研究所（批量导入）
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Benwei,Tan, Haidong,Qin, Yuqi,et al. Characterization of a new endo-type alginate lyase from Vibrio sp W13[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,75(APR):330-337.
APA Zhu, Benwei,Tan, Haidong,Qin, Yuqi,Xu, Qingsong,Du, Yuguang,&Yin, Heng.(2015).Characterization of a new endo-type alginate lyase from Vibrio sp W13.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,75(APR),330-337.
MLA Zhu, Benwei,et al."Characterization of a new endo-type alginate lyase from Vibrio sp W13".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 75.APR(2015):330-337.

入库方式： OAI收割

来源：过程工程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。