"Ready-to-use" hollow nanofiber membrane-based glucose testing strips
文献类型：期刊论文
| 作者 | Ji, Xiaoyuan1; Su, Zhiguo1,2; Wang, Ping1,3,4; Ma, Guanghui1; Zhang, Songping1,2 |
| 刊名 | ANALYST
 |
| 出版日期 | 2014-12-21 |
| 卷号 | 139期号:24页码:6467-6473 |
| 关键词 | ELECTROCHEMICAL BIOSENSORS ENZYME IMMOBILIZATION COMPOSITE NANOFIBERS GOLD NANOPARTICLES CARBON NANOFIBER OXIDASE ELECTRODE NANOCOMPOSITE NANOTUBE POLYMER |
| ISSN号 | 0003-2654 |
| 通讯作者 | Zhang, SP (reprint author), Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
| 英文摘要 | A novel "ready-to-use" glucose test strip based on a polyurethane hollow nanofiber membrane was fabricated through facile co-axial electrospinning. By utilizing glucose oxidase and horseradish peroxidase in the core-phase solution, and a chromogenic agent either in the core solution (in which case 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) was used) or in the shell-phase solution (in which case o-dianisidine was used) for co-axial electrospinning, in situ co-encapsulation of the two enzymes within the hollow nano-chamber and incorporation of chromogenic agents either inside the nano-chamber or in the shell of the hollow nanofibers was realized. Such unique "all-in-one" feature enabled the prepared hollow nanofiber membrane-based test strips to be applied either as colorimetric sensors in solution or as an optical biosensor operated in the "dip-and-read" mode. When used as a colorimetric biosensor in solution, the test strip with o-dianisidine as chromogenic agent shows an excellent linear response range between 0.01 mM to 20 mM and a high apparent lumped activity recovery of 62.1% as compared to the reaction rate of the free bi-enzyme system. While the activity recovery of the test strip with ABTS as chromogenic agent is only 18.0%, and the test strip is found to be unstable due to spontaneous-oxidation of the ABTS. The o-dianisidine test strip was also applied as an optical biosensor, visible rufous color was quickly developed on the surface of the membrane upon dropping 10 mu L of glucose sample, and an excellent correlation between differential diffusive reflectance of the test strip at 440 nm and glucose concentration was obtained in the range of 0.5-50 mM. The test strips also exhibited excellent long-term storage stability with a half-life at 25 degrees C as long as four months. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ELECTROCHEMICAL BIOSENSORS ; ENZYME IMMOBILIZATION ; COMPOSITE NANOFIBERS ; GOLD NANOPARTICLES ; CARBON NANOFIBER ; OXIDASE ; ELECTRODE ; NANOCOMPOSITE ; NANOTUBE ; POLYMER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345265300020 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/15243]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China 3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. "Ready-to-use" hollow nanofiber membrane-based glucose testing strips[J]. ANALYST,2014,139(24):6467-6473. |
| APA | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2014)."Ready-to-use" hollow nanofiber membrane-based glucose testing strips.ANALYST,139(24),6467-6473. |
| MLA | Ji, Xiaoyuan,et al.""Ready-to-use" hollow nanofiber membrane-based glucose testing strips".ANALYST 139.24(2014):6467-6473. |
入库方式： OAI收割
来源：过程工程研究所
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