Aptamer-Based Sensing Platform Using Three-Way DNA Junction-Driven Strand Displacement and Its Application in DNA Logic Circuit
文献类型：期刊论文
| 作者 | Zhu, Jinbo ; Zhang, Libing ; Zhou, Zhixue ; Dong,Shaojun ; Wang,Erkang |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:1页码:312-316 |
| 关键词 | SPLIT G-QUADRUPLEX COMPUTATION CASCADES MACHINE TOEHOLD NETWORK GATES |
| 通讯作者 | wang,ek |
| 英文摘要 | we proposed a new three-way dna junction-driven strand displacement mode and fabricated an aptamer-based label-free fluorescent sensing platform on the basis of this mechanism. assembling the aptamer sequence into the three-way dna junction makes the platform sensitive to the target of the aptamer. a label-free signal readout method, split g-quadruplex enhanced fluorescence of protoporphyrin ix (ppix), was used to report the final signal. here, adenosine triphosphatase (atp) was taken as a model and detected through this approach, and dna strand could also be detected by it. the mechanism was investigated by native polyacrylamide gel electrophoresis. furthermore, on the basis of this molecular platform, we built a logic circuit with atp and dna strands as input. aptamer played an important role in mediating the small molecule atp to tune the dna logic gate. through altering the aptamer sequence, this molecular platform will be sensitive to various stimuli and applied in a wide field. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329548700022 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/52381]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu, Jinbo,Zhang, Libing,Zhou, Zhixue,et al. Aptamer-Based Sensing Platform Using Three-Way DNA Junction-Driven Strand Displacement and Its Application in DNA Logic Circuit[J]. analytical chemistry,2014,86(1):312-316. |
| APA | Zhu, Jinbo,Zhang, Libing,Zhou, Zhixue,Dong,Shaojun,&Wang,Erkang.(2014).Aptamer-Based Sensing Platform Using Three-Way DNA Junction-Driven Strand Displacement and Its Application in DNA Logic Circuit.analytical chemistry,86(1),312-316. |
| MLA | Zhu, Jinbo,et al."Aptamer-Based Sensing Platform Using Three-Way DNA Junction-Driven Strand Displacement and Its Application in DNA Logic Circuit".analytical chemistry 86.1(2014):312-316. |
入库方式： OAI收割
来源：长春应用化学研究所
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