Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry
文献类型：期刊论文
| 作者 | Zheng, Yu ; Yang, Cheng ; Yang, Fan ; Yang,Xiurong |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:8页码:3849-3855 |
| 关键词 | CONFORMATIONAL-CHANGES PROTEIN ADSORPTION MULTILAYER FILMS IMMOBILIZATION HYBRIDIZATION BIOSENSOR CYSTEINE SPECTROSCOPY ANTIBODY BINDING |
| 通讯作者 | yang,f |
| 英文摘要 | the real-time conformational changes of cytosine (c)-rich ssdna oligonucleotides upon binding with silver ions (ag+) were studied using dual polarization interferometry (dpi). upon the addition of ag+, ag+ selectively bound to cytosine cytosine mismatches and formed c-ag+-c complexes, inducing change of the structure of the c-rich ssdna from random coil conformation to duplex conformation, whereas the control ssdna without cytosine-cytosine mismatches had no such signal, which was consistent with circular dichroism (cd) characterization. the conformational change of dna was reflected on the changes of the mass, thickness, and density values resolved by dpi. the calibration curves showed that as the concentration of ag+ increased from 10 nm to 8 mu m, the thickness and mass values increased linearly while the density values decreased linearly. other metal ions such as k+, ca2+, na+, mg2+, zn2+, mn2+, ni2+, and pb2+ did not interfere with the interaction between ag+ and c-rich ssdna, indicating that this method had a good selectivity. the practical application of this biosensor was also investigated in real samples such as drinking water. besides, cysteine could specifically capture ag+ from c-ag+-c complexes and transformed the structure of the c-rich dna back from rigid double-stranded conformation to random coil conformation, which allowed cysteine to be detected selectively as well. it is expected that this biosensing strategy may be utilized to study the interaction of dna with other molecules. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334658000026 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/52385]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zheng, Yu,Yang, Cheng,Yang, Fan,et al. Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry[J]. analytical chemistry,2014,86(8):3849-3855. |
| APA | Zheng, Yu,Yang, Cheng,Yang, Fan,&Yang,Xiurong.(2014).Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry.analytical chemistry,86(8),3849-3855. |
| MLA | Zheng, Yu,et al."Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry".analytical chemistry 86.8(2014):3849-3855. |
入库方式： OAI收割
来源：长春应用化学研究所
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