Polymer-Induced Perylene Probe Excimer Formation and Selective Sensing of DNA Methyltransferase Activity through the Monomer-Excimer Transition
文献类型：期刊论文
| 作者 | Wang, Yan ; Chen, Jian ; Chen, Yang ; Li,Wenying ; Yu,Cong |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:9页码:4371-4378 |
| 关键词 | TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE INITIATED ENZYMATIC POLYMERIZATION FREE COLORIMETRIC ASSAY ESCHERICHIA-COLI FLUORESCENCE DETECTION S-ADENOSYLMETHIONINE METHYLATION STRATEGY DYES CHEMILUMINESCENCE |
| 通讯作者 | yu,c |
| 英文摘要 | a new label-free strategy for sensitive fluorometric biosensing based on perylene probe monomer-excimer transition has been developed. a negatively charged perylene probe (compound 1) was used. compound 1 shows strong monomer fluorescence in an aqueous buffer solution. a cationic polymer could induce aggregation of compound 1 through noncovalent interactions. compound 1 monomer emission was quenched, and meanwhile strong excimer emission was observed. upon addition of a single-stranded dna (an anionic polymer), strong electrostatic attractive interactions between the cationic polymer and the dna weakened the binding of aggregates of compound 1 to the polycation. compound 1 monomers were released, and excimer monomer emission transition was detected. this observation formed the basis for dna methyltransferase (mtase) activity detection. when the 3'-oh terminus of a duplex dna was removed, the dna strands could not be elongated by terminal deoxynucleotidyl transferase (tdt), and little restoration of compound 1 monomer emission was detected. however, in the presence of mtase and endonuclease, the dna could be specifically methylated and then cleaved into single-stranded fragments. the dna fragments contained newly generated 3'-oh termini, which could be elongated by tdt. an excimer monomer transition signal could then be detected. simple, sensitive, selective, and inexpensive sensing of dna methylation was therefore established. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335719900044 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/52386]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Chen, Jian,Chen, Yang,et al. Polymer-Induced Perylene Probe Excimer Formation and Selective Sensing of DNA Methyltransferase Activity through the Monomer-Excimer Transition[J]. analytical chemistry,2014,86(9):4371-4378. |
| APA | Wang, Yan,Chen, Jian,Chen, Yang,Li,Wenying,&Yu,Cong.(2014).Polymer-Induced Perylene Probe Excimer Formation and Selective Sensing of DNA Methyltransferase Activity through the Monomer-Excimer Transition.analytical chemistry,86(9),4371-4378. |
| MLA | Wang, Yan,et al."Polymer-Induced Perylene Probe Excimer Formation and Selective Sensing of DNA Methyltransferase Activity through the Monomer-Excimer Transition".analytical chemistry 86.9(2014):4371-4378. |
入库方式： OAI收割
来源：长春应用化学研究所
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