Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters
文献类型：期刊论文
| 作者 | Sun, Jian ; Yang, Fan ; Zhao, Dan |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:15页码:7883-7889 |
| 关键词 | SELECTIVE DETECTION METAL NANOCLUSTERS QUANTUM DOTS COMPETITIVE COORDINATION PARATHYROID-HORMONE NANOPARTICLES CYSTEINE CLUSTERS PROBES CU2+ |
| 通讯作者 | yang,xr |
| 英文摘要 | on the basis of the competition assay approach and fluorescent 11-mercaptoundecanoic acid-capped auncs (auncs@11-mua) with unique optical properties, a convenient, reliable and highly sensitive real-time assay of pyrophosphatase (ppase) activity is established and developed for the first time. pyrophosphate (pp could recover the cu2+-quenched aunc5@11-mua fluorescence selectively owing to the higher binding affinity between ppi and cu2+ than that between 11-mua and cu2+. whereas ppase could catalyze the hydrolysis of ppi, thus released cu2+, leading to fluorescence requenching of the auncs@11-mua. in the assay, a good linearity between the fluorescence response and ppase activity within a range from 1 to 20 mu is found, with a detection limit of less than 1 mu, which is better than other ppase assays using ppi as the substrate. additionally, we demonstrate that our auncs@11-mua-based fluorescent assay can be applied to assay the ppase activity in real biological samples such as the cell lysate. this strategy paves a new avenue for exploring the sensing applications of fluorescence auncs and improving the development of competition assay approach. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340081100101 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/52390]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun, Jian,Yang, Fan,Zhao, Dan. Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters[J]. analytical chemistry,2014,86(15):7883-7889. |
| APA | Sun, Jian,Yang, Fan,&Zhao, Dan.(2014).Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters.analytical chemistry,86(15),7883-7889. |
| MLA | Sun, Jian,et al."Highly Sensitive Real-Time Assay of Inorganic Pyrophosphatase Activity Based on the Fluorescent Gold Nanoclusters".analytical chemistry 86.15(2014):7883-7889. |
入库方式： OAI收割
来源：长春应用化学研究所
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