Identification of Unfolding and Dissociation Pathways of Superoxide Dismutase in the Gas Phase by Ion-Mobility Separation and Tandem Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhuang, Xiaoyu ; Liu, Shu ; Zhang, Ruixing ; Song,Fengrui ; Liu,Zhiqiang ; Liu,Shuying |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:23页码:11599-11605 |
| 关键词 | AMYOTROPHIC-LATERAL-SCLEROSIS CHARGED PROTEIN COMPLEXES ELECTROSPRAY-IONIZATION MACROMOLECULAR ASSEMBLIES STRUCTURAL BIOLOGY 1 SOD-1 PRESERVATION CONFORMATION AGGREGATION HOMODIMERS |
| 通讯作者 | song,fr |
| 英文摘要 | cu, zn-superoxide dismutase (sod1) is a homodimeric enzyme of approximately 32 kda. each monomer contains one cu2+ and one zn2+ ion, which play catalytic and structural roles in the enzyme. dimer formation is also essential to its functionality. the spatial structure of this metalloenzyme is also closely related to its bioactivities. here we investigate the structural and conformational changes of sod1 in the gas phase by electrospray ionization mass spectrometry (esi-ms) and ion-mobility (im) separation combined with tandem mass spectrometry (ms/ms). first, the composition and forms of sod1 were analyzed by esi-ms. the dimer, monomer, and apomonomer were observed under different solvent conditions. the dimer was found to be stable, and could retain its native structure in neutral buffer. ion-mobility separation combined with ms/ms was used to reveal the conformational changes and dissociation process of sod1 when it was activated in the gas phase. three different dimeric and two monomeric conformers were observed; three unfolding and dissociation pathways were also identified. the results from this study demonstrate that im-ms/ms could be used to obtain spatial structural information on sod1 and that the technique could therefore be employed to investigate the conformational changes in mutant sod1, which is related to amyotrophic lateral sclerosis and other neurodegenerative disorders. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345803300022 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/52395]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhuang, Xiaoyu,Liu, Shu,Zhang, Ruixing,et al. Identification of Unfolding and Dissociation Pathways of Superoxide Dismutase in the Gas Phase by Ion-Mobility Separation and Tandem Mass Spectrometry[J]. analytical chemistry,2014,86(23):11599-11605. |
| APA | Zhuang, Xiaoyu,Liu, Shu,Zhang, Ruixing,Song,Fengrui,Liu,Zhiqiang,&Liu,Shuying.(2014).Identification of Unfolding and Dissociation Pathways of Superoxide Dismutase in the Gas Phase by Ion-Mobility Separation and Tandem Mass Spectrometry.analytical chemistry,86(23),11599-11605. |
| MLA | Zhuang, Xiaoyu,et al."Identification of Unfolding and Dissociation Pathways of Superoxide Dismutase in the Gas Phase by Ion-Mobility Separation and Tandem Mass Spectrometry".analytical chemistry 86.23(2014):11599-11605. |
入库方式： OAI收割
来源：长春应用化学研究所
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