A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity
文献类型：期刊论文
| 作者 | Qi, Peng1,2 ; Zhang, Dun1; Wan, Yi1
|
| 刊名 | TALANTA
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 129页码:270-275 |
| 关键词 | Sulfate-reducing bacteria Cysteine protease S-sulfhydration Inhibition |
| 通讯作者 | Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Sulfate-reducing bacteria (SRB) have been extensively studied in corrosion and environmental science. However, fast enumeration of SRB population is still a difficult task. This work presents a novel specific SRB detection method based on inhibition of cysteine protease activity. The hydrolytic activity of cysteine protease was inhibited by taking advantage of sulfide, the characteristic metabolic product of SRB, to attack active cysteine thiol group in cysteine protease catalytic sites. The active thiol S-sulfhydration process could be used for SRB detection, since the amount of sulfide accumulated in culture medium was highly related with initial bacterial concentration. The working conditions of cysteine protease have been optimized to obtain better detection capability, and the SRB detection performances have been evaluated in this work. The proposed SRB detection method based on inhibition of cysteine protease activity avoided the use of biological recognition elements. In addition, compared with the widely used most probable number (MPN) method which would take up to at least 15 days to accomplish whole detection process, the method based on inhibition of papain activity could detect SRB in 2 days, with a detection limit of 5.21 x 10(2) cfu mL(-1). The detection time for SRB population quantitative analysis was greatly shortened. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341463400038 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/23855]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Peng,Zhang, Dun,Wan, Yi. A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity[J]. TALANTA,2014,129:270-275. |
| APA | Qi, Peng,Zhang, Dun,&Wan, Yi.(2014).A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity.TALANTA,129,270-275. |
| MLA | Qi, Peng,et al."A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity".TALANTA 129(2014):270-275. |
入库方式： OAI收割
来源：海洋研究所
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