Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria
文献类型：期刊论文
| 作者 | Wan, Yi1; Sun, Yan1; Qi, Peng1,2 ; Wang, Peng1; Zhang, Dun1
|
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2014-05-15 |
| 卷号 | 55页码:289-293 |
| 关键词 | Quatemized magnetic nanoparticles Bacteria detection Fluorescent polymer Linear discriminant analysis |
| 通讯作者 | Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Nanomaterial-based 'chemical nose' sensor with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. A sensor array containing three quaternized magnetic nanoparticles (q-MNPs)-fluorescent polymer systems has been designed to identify and quantify bacteria. The bacterial cell membranes disrupt the q-MNP-fluorescent polymer, generating unique fluorescence response array. The response intensity of the array is dependent on the level of displacement determined by the relative q-MNP-fluorescent polymer binding strength and bacteria cells-MNP interaction. These characteristic responses show a highly repeatable bacteria cells and can be differentiated by linear discriminant analysis (LDA). Based on the array response matrix from LDA, our approach has been used to measure bacteria with an accuracy of 87.5% for 107 cfu mL-1 within 20 min. Combined with UV-vis measurement, the method can be successfully performed to identify and detect eight different pathogen samples with an accuracy of 96.8%. The measurement system has a potential for further applications and provides a facile and simple method for the rapid analysis of protein, DNA, and pathogens. (C) 2013 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332426100046 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/23885]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Sun, Yan,Qi, Peng,et al. Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria[J]. BIOSENSORS & BIOELECTRONICS,2014,55:289-293. |
| APA | Wan, Yi,Sun, Yan,Qi, Peng,Wang, Peng,&Zhang, Dun.(2014).Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria.BIOSENSORS & BIOELECTRONICS,55,289-293. |
| MLA | Wan, Yi,et al."Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria".BIOSENSORS & BIOELECTRONICS 55(2014):289-293. |
入库方式： OAI收割
来源：海洋研究所
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