Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
文献类型：期刊论文
| 作者 | Wang, Guoshan1; Yu, Zhigang2,3; Zhen, Yu4,5; Mi, Tiezhu4,5; Shi, Yan5; Wang, Jianyan1; Wang, Minxiao6; Sun, Song6
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-06-13 |
| 卷号 | 9期号:6页码:e100057 |
| 通讯作者 | Zhen, Y (reprint author), Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Shandong, Peoples R China. |
| 英文摘要 | The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earliest eumetazoans, Cnidarians can be served as an interesting model for exploring the HIF system from an evolutionary perspective. We identified the complete cDNA sequence of HIF-1 alpha (ASHIF) from the Aurelia sp.1, and the predicted HIF-1 alpha protein (pASHIF) was comprised of 674 amino acids originating from 2,025 bp nucleotides. A Pairwise comparison revealed that pASHIF not only possessed conserved basic helix-loop-helix (bHLH) and Per-Arnt-Sim (PAS) domains but also contained the oxygen dependent degradation (ODD) and the C-terminal transactivation domains (C-TAD), the key domains for hypoxia regulation. As indicated by sequence analysis, the ASHIF gene contains 8 exons interrupted by 7 introns. Western blot analysis indicated that pASHIF that existed in the polyps and medusa of Aurelia. sp.1 was more stable for a hypoxic response than normoxia. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338278100112 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24028]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China 2.Minist Educ, Key Lab Marine Chem Theory & Engn, Qingdao, Shandong, Peoples R China 3.Ocean Univ China, Coll Chem & Chem Engn, Qingdao, Shandong, Peoples R China 4.Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Shandong, Peoples R China 5.Ocean Univ China, Coll Environm Sci & Engn, Qingdao, Shandong, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Guoshan,Yu, Zhigang,Zhen, Yu,et al. Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1[J]. PLOS ONE,2014,9(6):e100057. |
| APA | Wang, Guoshan.,Yu, Zhigang.,Zhen, Yu.,Mi, Tiezhu.,Shi, Yan.,...&Sun, Song.(2014).Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1.PLOS ONE,9(6),e100057. |
| MLA | Wang, Guoshan,et al."Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1".PLOS ONE 9.6(2014):e100057. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。