Transcription profiling using RNA-Seq demonstrates expression differences in the body walls of juvenile albino and normal sea cucumbers Apostichopus japonicus
文献类型：期刊论文
| 作者 | Ma Deyou1,2; Yang Hongsheng1 ; Sun Lina1,2; Chen Muyan3
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| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 32期号:1页码:34-46 |
| 关键词 | albino gene expression profiling RNA-Seq real-time PCR Apostichopus japonicus |
| 通讯作者 | Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China. |
| 英文摘要 | Sea cucumbers Apostichopus japonicus are one of the most important aquaculture species in China. Their normal body color is black to fit their surroundings. Wild albinos are rare and hard to breed. To understand the differences between albino and normal (control) sea cucumbers at the transcriptional level, we sequenced the transcriptomes in their body-wall tissues using RNA-Seq high-throughput sequencing. Approximately 4.876 million (M) and 4.884 M 200-nucleotide-long cDNA reads were produced in the cDNA libraries derived from the body walls of albino and control samples, respectively. A total of 9 561 (46.89%) putative genes were identified from among the RNA-Seq reads in both libraries. After filtering, 837 significantly differentially regulated genes were identified in the albino library compared with in the control library, and 3.6% of the differentially expressed genes (DEGs) were found to have changed those more than five-fold. The expression levels of 10 DEGs were checked by real-time PCR and the results were in full accord with the RNA-Seq expression trends, although the amplitude of the differences in expression levels was lower in all cases. A series of pathways were significantly enriched for the DEGs. These pathways were closely related to phagocytosis, the complement and coagulation cascades, apoptosis-related diseases, cytokine-cytokine receptor interaction, and cell adhesion. The differences in gene expression and enriched pathways between the albino and control sea cucumbers offer control targets for cultivating excellent albino A. japonicus strains in the future. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332003500004 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24052]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Ocean Univ China, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma Deyou,Yang Hongsheng,Sun Lina,et al. Transcription profiling using RNA-Seq demonstrates expression differences in the body walls of juvenile albino and normal sea cucumbers Apostichopus japonicus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(1):34-46. |
| APA | Ma Deyou,Yang Hongsheng,Sun Lina,&Chen Muyan.(2014).Transcription profiling using RNA-Seq demonstrates expression differences in the body walls of juvenile albino and normal sea cucumbers Apostichopus japonicus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(1),34-46. |
| MLA | Ma Deyou,et al."Transcription profiling using RNA-Seq demonstrates expression differences in the body walls of juvenile albino and normal sea cucumbers Apostichopus japonicus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.1(2014):34-46. |
入库方式： OAI收割
来源：海洋研究所
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