Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics
文献类型：期刊论文
| 作者 | Cheng Fangping1,2; Wang Minxiao1; Li Chaolun1 ; Sun Song1
|
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 32期号:4页码:858-870 |
| 关键词 | zooplankton DNA barcodes cytochrome oxidase subunit 1 (cox1) |
| 通讯作者 | Sun, S (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China. |
| 英文摘要 | DNA barcoding provides accurate identification of zooplankton species through all life stages. Single-gene-targeted metagenomic analysis based on DNA barcode databases can facilitate long-term monitoring of zooplankton communities. With the help of the available zooplankton databases, the zooplankton community of the Changjiang (Yangtze) River estuary was studied using a single-gene-targeted metagenomic method to estimate the species richness of this community. A total of 856 mitochondrial cytochrome oxidase subunit 1 (cox1) gene sequences were determined. The environmental barcodes were clustered into 70 molecular operational taxonomic units (MOTUs). Forty-two MOTUs matched barcoded marine organisms with more than 90% similarity and were assigned to either the species (similarity>96%) or genus level (similarity<96%). Sibling species could also be distinguished. Many species that were overlooked by morphological methods were identified by molecular methods, especially gelatinous zooplankton and merozooplankton that were likely sampled at different life history phases. Zooplankton community structures differed significantly among all of the samples. The MOTU spatial distributions were influenced by the ecological habits of the corresponding species. In conclusion, single-gene-targeted metagenomic analysis is a useful tool for zooplankton studies, with which specimens from all life history stages can be identified quickly and effectively with a comprehensive database. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339046200013 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24055]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng Fangping,Wang Minxiao,Li Chaolun,et al. Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(4):858-870. |
| APA | Cheng Fangping,Wang Minxiao,Li Chaolun,&Sun Song.(2014).Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(4),858-870. |
| MLA | Cheng Fangping,et al."Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.4(2014):858-870. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。