SNP Identification by Transcriptome Sequencing and Candidate Gene-Based Association Analysis for Heat Tolerance in the Bay Scallop Argopecten irradians
文献类型：期刊论文
| 作者 | Du, Xuedi1,2 ; Li, Li1; Zhang, Shoudu1; Meng, Fei3; Zhang, Guofan1
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-08-14 |
| 卷号 | 9期号:8页码:e104960 |
| 通讯作者 | 张国范 |
| 英文摘要 | The northern bay scallop Argopecten irradians irradians (Lamarck) and the southern bay scallop Argopecten irradians concentricus (Say) were introduced into China in the 1980s and 1990s, and are now major aquaculture molluscs in China. Here, we report the transcriptome sequencing of the two subspecies and the subsequent association analysis on candidate gene on the trait of heat tolerance. In total, RNA from six tissues of 67 and 42 individuals of northern and southern bay scallops, respectively, were used and 55.5 and 34.9 million raw reads were generated, respectively. There were 82,267 unigenes produced in total, of which 32,595 were annotated. Altogether, 32,206 and 23,312 high-quality SNPs were identified for northern and southern bay scallops, respectively. For case-control analysis, two intercrossed populations were heat stress treated, and both heat-susceptible and heat-resistant individuals were collected. According to annotation and SNP allele frequency analysis, 476 unigenes were selected, and 399 pairs of primers were designed. Genotyping was conducted using the high-resolution melting method, and Fisher's exact test was performed for allele frequency comparison between the heat-susceptible and heat-resistant groups. SNP all-53308-760 T/C showed a significant difference in allele frequency between the heat-susceptible and heat-resistant groups. Notably, considerable difference in allele frequency at this locus was also observed between the sequenced natural populations. These results suggest that SNP all-53308- 760 T/C may be related to the heat tolerance of the bay scallop. Moreover, quantitative expression analysis revealed that the expression level of all-53308 was negatively correlated with heat tolerance of the bay scallop. |
| 学科主题 | 海洋生物学 ; 海洋生物技术 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341017000079 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/22792]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Biomarker Technol Corp, Dept Informat Anal, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Xuedi,Li, Li,Zhang, Shoudu,et al. SNP Identification by Transcriptome Sequencing and Candidate Gene-Based Association Analysis for Heat Tolerance in the Bay Scallop Argopecten irradians[J]. PLOS ONE,2014,9(8):e104960. |
| APA | Du, Xuedi,Li, Li,Zhang, Shoudu,Meng, Fei,&Zhang, Guofan.(2014).SNP Identification by Transcriptome Sequencing and Candidate Gene-Based Association Analysis for Heat Tolerance in the Bay Scallop Argopecten irradians.PLOS ONE,9(8),e104960. |
| MLA | Du, Xuedi,et al."SNP Identification by Transcriptome Sequencing and Candidate Gene-Based Association Analysis for Heat Tolerance in the Bay Scallop Argopecten irradians".PLOS ONE 9.8(2014):e104960. |
入库方式： OAI收割
来源：海洋研究所
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