Isochrysis sp IOAC724S, a newly isolated, lipid-enriched, marine microalga for lipid production, and optimized cultivation conditions
文献类型：期刊论文
| 作者 | Liu, Qian ; Pang, Tong; Li, Ling; Liu, Jianguo; Lin, Wei
|
| 刊名 | BIOMASS & BIOENERGY
 |
| 出版日期 | 2014 |
| 卷号 | 60页码:32-40 |
| 关键词 | Marine microalgae Isochrysis sp IOAC724S High temperature tolerance Fatty acid Lipid Optimization |
| 通讯作者 | Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao, Peoples R China. |
| 英文摘要 | An oleaginous, unicellular, marine microalga termed IOAC724S was isolated from the South China Sea. Morphology and genetic analyses indicated it belongs to the genus Isochrysis. Gas chromatography (GC) results showed that more than 10 types of fatty acids existed in Isochrysis sp. IOAC724S and that 90% of them were suitable for lipid production. The culture conditions suitable for cell growth were progressively optimized through photosynthetic and respiratory analyses. The optimal culture conditions were: photon flux 200-500 mu mol m (2-) s (1), temperature 35 degrees C during daytime and 24 degrees C at night, pH value between 7 and 8, NaNO3 160 g m (-3) and NaH2PO4 center dot 2H(2)O 80 g m(-3) for starting culture. When microalgal cultures were exposed to these optimal conditions, the specific growth rate reached to 0.26 d (1) on average and 1.0 d(-1) in MAX. Lipid production was optimized through nutrient starvation processes, including nitrate or phosphate deprivation and simultaneous nitrate and phosphate deprivation. The highest lipid mass fraction of dry cell weight (about 55.6%) was obtained after the stationary phase algal culture was transferred into phosphate-free medium for 3 days. GC data demonstrated that the enhancement of lipid accumulation in algal cells maintained under nutrient starvation came mainly from an increase of C16:0 and C18:1 fatty acids; however, the lipids with a chain length appropriate for fuel use (C14 to C18) were unchanged at 90% mass fraction of the dry cell weight. Based on these good characteristics, Isochrysis sp. IOAC724S appeared to be a strong candidate for lipid production. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330824100004 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24228]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_文献信息中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qian,Pang, Tong,Li, Ling,et al. Isochrysis sp IOAC724S, a newly isolated, lipid-enriched, marine microalga for lipid production, and optimized cultivation conditions[J]. BIOMASS & BIOENERGY,2014,60:32-40. |
| APA | Liu, Qian,Pang, Tong,Li, Ling,Liu, Jianguo,&Lin, Wei.(2014).Isochrysis sp IOAC724S, a newly isolated, lipid-enriched, marine microalga for lipid production, and optimized cultivation conditions.BIOMASS & BIOENERGY,60,32-40. |
| MLA | Liu, Qian,et al."Isochrysis sp IOAC724S, a newly isolated, lipid-enriched, marine microalga for lipid production, and optimized cultivation conditions".BIOMASS & BIOENERGY 60(2014):32-40. |
入库方式： OAI收割
来源：海洋研究所
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