A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri
文献类型：期刊论文
| 作者 | Yue, Feng1,2; Zhou, Zhi1; Wang, Lingling1; Wang, Mengqiang1 ; Song, Linsheng1
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 39期号:2页码:125-135 |
| 关键词 | Chlamys farreri Transcription factor GATA Hemocyte production Hematopoiesis |
| 通讯作者 | Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China. |
| 英文摘要 | GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence "GATA", and involved in a myriad of cellular processes. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. The full-length cDNA of CfGATA is of 2058 bp encoding a predicted polypeptide of 457 amino acids with two conserved zinc finger domains, which shared high similarity with other reported GATA1/2/3 proteins. The mRNA transcripts of CfGATA showed higher expression in gills, hepatopancreas, hemocytes and heart, and the CfGATA protein expressed in HEK293 cells was found to be localized specifically in the nuclei. The recombinant CfGATA protein (rCfGATA) exhibited strong ability to bind specific WGATAR DNA sequence by electrophoretic mobility shift assay in vitro. After CfGATA gene was silenced by RNA interference, the hemocyte renewal rate and circulating total hemocyte count (THC) decreased significantly, which was 7.85-fold and 19.46-fold lower than that of PBS control, respectively (P < 0.05). After LPS stimulation, the expression level of CfGATA mRNA decreased significantly in the hemocytes of PBS or EGFP dsRNA treated scallops, which was accompanied by the increase of hemocyte renewal rate and the reduced circulating THC at 24 h. In contrast, the hemocyte renewal rate and circulating THC did not change significantly in CfGATA gene interfered scallops after LPS stimulation. These results suggested that CfGATA, as a conserved GATA1/2/3 transcription factor, plays essential roles in regulating hemocyte production of scallop. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339773000001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24056]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Feng,Zhou, Zhi,Wang, Lingling,et al. A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):125-135. |
| APA | Yue, Feng,Zhou, Zhi,Wang, Lingling,Wang, Mengqiang,&Song, Linsheng.(2014).A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,39(2),125-135. |
| MLA | Yue, Feng,et al."A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):125-135. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。