中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions

文献类型:期刊论文

作者Zhang, Jiquan1; Wang, Jing1,2; Gui, Tianshu1,2; Sun, Zheng1; Xiang, Jianhai1
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2014-09-01
卷号70页码:246-250
关键词Metallothionein E. carinicauda Developmental stage
通讯作者Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要A full-length copper-induced metallothionein (EcMT-Cu) cDNA was obtained from Exopalaemon carinicauda (Holthuis) and it contained a 198bp open reading frame that encoded a peptide with 65 amino acid residues. Twenty-one cysteines were found in deduced amino acid sequence and the cysteine (Cys)-rich characteristic was also reported in different types of metallothioneins from other species. EcMT-Cu mRNA expression profile showed that it is the hepatopancreas specific gene. The expression of EcMT-Cu was extremely different when shrimp were exposed to seawater containing 50 mu M CuSO4 or 2.5 mu M CdCl2. The expression of EcMT-Cu in shrimp was significantly up-regulated at 12 and 24h after exposure to CuSO4, however, its expression was not induced compared to that of pretreatment (p > 0.05) when shrimp were exposed to CdCl2. The transcript of EcMT-Cu was found to be extremely low at gastrula and nauplius stage and expression of EcMT-Cu could be detected from egg protozoa stage. (C) 2014 Elsevier B.V. All rights reserved.
学科主题Biochemistry & Molecular Biology
收录类别SCI
语种英语
WOS记录号WOS:000342478800035
源URL[http://ir.qdio.ac.cn/handle/337002/24057]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jiquan,Wang, Jing,Gui, Tianshu,et al. A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,70:246-250.
APA Zhang, Jiquan,Wang, Jing,Gui, Tianshu,Sun, Zheng,&Xiang, Jianhai.(2014).A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,70,246-250.
MLA Zhang, Jiquan,et al."A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 70(2014):246-250.

入库方式: OAI收割

来源:海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。