A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)
文献类型：期刊论文
| 作者 | Deng, Yunyan1; Wang, Xiuliang1; Guo, Hui1,2; Duan, Delin1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 41期号:1页码:529-536 |
| 关键词 | CBM20 domain Drought adaption Saccharina japonica Trehalose-6-phosphate synthase TPS domain TPP domain |
| 通讯作者 | Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | The full-length cDNA sequence of a trehalose-6-phosphate synthase gene from Saccharina japonica (designated as SjaTPS) (Accession: KC578568) was isolated based on homologous cloning and RACE-PCR. It was 4,127 bp, with 320 bp 5'-UTR, 21 bp 3'-UTR, and open reading frame (ORF) of 3,786 bp. The deduced 1,261 amino acids characterized with predicted molecular weight of 137.84 kDa and theoretical isoelectric point of 7.12. The SjaTPS had one N-terminal CBM20 (family 20 carbohydrate-binding module) domain, one TPS domain (trehalose-6-phosphate synthase) in the middle region and a single TPP (trehalose-6-phosphate phosphatase) domain near the C-terminus. Structural analysis suggested that the SjaTPS putatively functioned as trehalose-6-phosphate synthase, and might be related to laminaran metabolism in S. japonica. Homology analysis indicated that the SjaTPS shared 49-70 % similarities with the 13 known TPS sequences of other algae; only 55 % amino acid similarities were detected between SjaTPS and the previously reported TPS sequence of S. japonica (Accession: DQ666325). Phylogenetic analysis revealed close affinity between SjaTPS and TPS of brown alga Ectocarpus siliculosus (Accession: CBJ29609). Transcriptional analysis showed that desiccation greatly enhanced SjaTPS expression and the maximum appeared at 3 h, which was about 300-fold compared to that of the start, implied that SjaTPS was involved with drought adaption in kelp. In vitro expression of SjaTPS showed that one distinct band existed at similar to 115 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results increased the knowledge of trehalose-6-phosphate synthase properties in S. japonica and also important for better understanding the role trehalose plays in kelp abiotic tolerance for adaption to the sublittoral habitats. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329100500057 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24064]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Yunyan,Wang, Xiuliang,Guo, Hui,et al. A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)[J]. MOLECULAR BIOLOGY REPORTS,2014,41(1):529-536. |
| APA | Deng, Yunyan,Wang, Xiuliang,Guo, Hui,&Duan, Delin.(2014).A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae).MOLECULAR BIOLOGY REPORTS,41(1),529-536. |
| MLA | Deng, Yunyan,et al."A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)".MOLECULAR BIOLOGY REPORTS 41.1(2014):529-536. |
入库方式： OAI收割
来源：海洋研究所
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