Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Wen, Rong1,2; Li, Fuhua1; Xie, Yusu1; Li, Shihao1; Zhang, Chengsong1 ; Yu, Kuijie1; Xiang, Jianhai1
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| 刊名 | INVERTEBRATE REPRODUCTION & DEVELOPMENT
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 58期号:3页码:226-234 |
| 关键词 | ovary testis development triploid penaeid shrimp |
| 通讯作者 | Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 英文摘要 | The spermatogonial stem-cell renewal factor (SSRF) was named since its function in spermatogonial mitosis was reported in Japanese eel. Our previous study showed that a homolog of SSRF was highly expressed in the ovary of triploid shrimp, but not expressed in the ovary of diploid shrimp. To understand the function of SSRF in shrimp, the full-length cDNA of ssrf gene was cloned from Chinese shrimp Fenneropenaeus chinensis (Fcssrf) and its expression was analyzed. The full length of Fcssrf cDNA was 2588 bp and it contained an open reading frame encoding 450 amino acids. The predicted tertiary structure of FcSSRF was very similar to that of SSRF/eSRS34 from Anguilla japonica and TP/PD-ECGF from Homo sapiens. RT-PCR analysis showed that the Fcssrf was highly expressed in nerve, testis, hepatopancreas, gill, and stomach rather than in ovary. Expression of Fcssrf mRNA was not detected during embryonic stages and larval stages, from the nauplii to the post-larvae stage, in diploid, and triploid shrimp. However, it began to be expressed in juvenile stages (June-September) in diploid and triploid shrimp. Immunohistochemical analyses showed that FcSSRF was identified in both the diploid testis and triploid ovary. We inferred that the Fcssrf might be related to testis development. |
| 学科主题 | Reproductive Biology; Zoology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338105700009 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24088]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Rong,Li, Fuhua,Xie, Yusu,et al. Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2014,58(3):226-234. |
| APA | Wen, Rong.,Li, Fuhua.,Xie, Yusu.,Li, Shihao.,Zhang, Chengsong.,...&Xiang, Jianhai.(2014).Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis.INVERTEBRATE REPRODUCTION & DEVELOPMENT,58(3),226-234. |
| MLA | Wen, Rong,et al."Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis".INVERTEBRATE REPRODUCTION & DEVELOPMENT 58.3(2014):226-234. |
入库方式： OAI收割
来源：海洋研究所
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